Direct and indirect Vitamin A supplementation strategies result in different plasma and tissue retinol kinetics in neonatal rats

Libo Tan, Amanda E. Babbs, Michael H. Green, A. Catharine Ross

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Many questions remain regarding vitamin A (VA) supplementation of infants. Herein we compared direct oral VA supplementation of the neonate and indirect treatment through maternal dietary VA (M-VA) treatment on VA status and kinetics in neonatal rats. Treatments included direct VA combined with retinoic acid (RA) [D-VARA; VA (6 mg/kg) + 10% RA, given orally to neonates on postnatal day (P)2 and P3] and indirect VA supplementation through increased M-VA, compared with each other and oil-treated neonates. [3 H]retinol was administered orally to all neonates on P4. Plasma and tissue [3 H]retinol kinetics were determined from 1 h to 14 days post-dosing. D-VARA versus placebo dramatically increased liver and lung retinol, but only in the first 8-10 days. In M-VA neonates, liver and lung VA increased progressively throughout the study. Compartmental modeling of plasma [3 H]retinol showed that both D-VARA and indirect M-VA reduced retinol recycling between plasma and tissues. Compartmental models of individual tissues predicted that D-VARA stimulated the uptake of VA in chylomicrons to extrahepatic tissues, especially intestine, while the uptake was not observed in M-VA neonates. In conclusion, indirect maternal supplementation had a greater sustained effect than D-VARA on neonatal VA status, while also differentially affecting plasma and tissue retinol kinetics.-Tan, L., A. E. Babbs, M. H. Green, and A. C. Ross. Direct and indirect vitamin A supplementation strategies result in different plasma and tissue retinol kinetics in neonatal rats. J. Lipid Res. 2016. 57: 1423-1434.

Original languageEnglish (US)
Pages (from-to)1423-1434
Number of pages12
JournalJournal of Lipid Research
Volume57
Issue number8
DOIs
StatePublished - Aug 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

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