Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification

M. R. Andersen, Curtis John Omiecinski

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this report we describe a simple and rapid technique using DNA affinity columns that permits direct extraction of bacterial plasmids from a variety of foods for polymerase chain reaction amplification. The procedure was used to detect virulent enteroinvasive Escherichia coli in several artificially seeded matrices, including seafoods, greens, dairy products, enrichment media, and water. Polymerase inhibitors present in both foods and enrichment media were removed efficiently.

Original languageEnglish (US)
Pages (from-to)4080-4082
Number of pages3
JournalApplied and Environmental Microbiology
Volume58
Issue number12
StatePublished - Jan 1 1992

Fingerprint

enteroinvasive Escherichia coli
Seafood
Food Chain
Dairy Products
seafoods
plasmid
polymerase chain reaction
dairy products
amplification
plasmids
foods
Plasmids
Escherichia coli
Food
Polymerase Chain Reaction
food
Water
seafood
DNA
inhibitor

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Andersen, M. R. ; Omiecinski, Curtis John. / Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification. In: Applied and Environmental Microbiology. 1992 ; Vol. 58, No. 12. pp. 4080-4082.
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Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification. / Andersen, M. R.; Omiecinski, Curtis John.

In: Applied and Environmental Microbiology, Vol. 58, No. 12, 01.01.1992, p. 4080-4082.

Research output: Contribution to journalArticle

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