Direct measurement of 1H-1H dipolar couplings in proteins: A complement to traditional NOE measurements

Fang Tian, C. A. Fowler, E. R. Zartler, Jr Jenney, M. W. Adams, J. H. Prestegard

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

An intensity-based constant-time COSY (CT-COSY) method is described for measuring 1H-1H residual dipolar couplings of proteins in weakly aligned media. For small proteins, the overall sensitivity of this experiment is comparable to the NOESY experiment. In cases where the 1H-1H distances are defined by secondary structure, such as 1H(α)-1H(N) and 1H(N)-1H(N) sequential distances in α-helices and β-sheets, these measurements provide useful orientational constraints for protein structure determination. This experiment can also be used to provide distance information similar to that obtained from NOE connectivities once the angular dependence is removed. Because the measurements are direct and non-coherent processes, such as spin diffusion, do not enter, the measurements can be more reliable. The 1/r3 distance dependence of directly observed dipolar couplings, as compared with the 1/r6 distance dependence of NOEs, also can provide longer range distance information at favorable angles. A simple 3D, 15N resolved version of the pulse sequence extends the method to provide the improved resolution required for application to larger biomolecules.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalJournal of Biomolecular NMR
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2000

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Staphylococcal Protein A
Proteins
Experiments
Biomolecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Spectroscopy

Cite this

Tian, Fang ; Fowler, C. A. ; Zartler, E. R. ; Jenney, Jr ; Adams, M. W. ; Prestegard, J. H. / Direct measurement of 1H-1H dipolar couplings in proteins : A complement to traditional NOE measurements. In: Journal of Biomolecular NMR. 2000 ; Vol. 18, No. 1. pp. 23-31.
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Direct measurement of 1H-1H dipolar couplings in proteins : A complement to traditional NOE measurements. / Tian, Fang; Fowler, C. A.; Zartler, E. R.; Jenney, Jr; Adams, M. W.; Prestegard, J. H.

In: Journal of Biomolecular NMR, Vol. 18, No. 1, 01.01.2000, p. 23-31.

Research output: Contribution to journalArticle

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