Direct phosphorylation and activation of a mitogen-activated protein kinase by a calcium-dependent protein kinase in rice

Kabin Xie, Jianping Chen, Qin Wang, Yinong Yang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The mitogen-activated protein kinase (MAPK) is a pivotal point of convergence for many signaling pathways in eukaryotes. In the classical MAPK cascade, a signal is transmitted via sequential phosphorylation and activation of MAPK kinase kinase, MAPK kinase (MKK), and MAPK. The activation of MAPK is dependent on dual phosphorylation of a TXY motif by an MKK, which is considered the sole kinase to phosphorylate and activate MAPK. Here, we report a novel regulatory mechanism of MAPK phosphorylation and activation besides the canonical MAPK cascade. A rice (Oryza sativa) calcium-dependent protein kinase (CDPK), CPK18, was identified as an upstream kinase of MAPK (MPK5) in vitro and in vivo. Curiously, CPK18 was shown to phosphorylate and activate MPK5 without affecting the phosphorylation of its TXY motif. Instead, CPK18 was found to predominantly phosphorylate two Thr residues (Thr-14 and Thr-32) that are widely conserved in MAPKs from land plants. Further analyses reveal that the newly identified CPK18-MPK5 pathway represses defense gene expression and negatively regulates rice blast resistance. Our results suggest that land plants have evolved an MKK-independent phosphorylation pathway that directly connects calcium signaling to the MAPK machinery.

Original languageEnglish (US)
Pages (from-to)3077-3089
Number of pages13
JournalPlant Cell
Volume26
Issue number7
DOIs
StatePublished - Jul 2014

Fingerprint

Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
protein kinases
phosphorylation
Phosphorylation
MAP Kinase Kinase Kinases
calcium
rice
mitogen-activated protein kinase kinase kinase
mitogen-activated protein kinase kinase
Embryophyta
Mitogen-Activated Protein Kinase Kinases
embryophytes
Phosphotransferases
blast disease
Calcium Signaling
rice calcium-dependent protein kinase
Eukaryota
eukaryotic cells
Oryza sativa

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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Direct phosphorylation and activation of a mitogen-activated protein kinase by a calcium-dependent protein kinase in rice. / Xie, Kabin; Chen, Jianping; Wang, Qin; Yang, Yinong.

In: Plant Cell, Vol. 26, No. 7, 07.2014, p. 3077-3089.

Research output: Contribution to journalArticle

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