Abstract
The 2-μm circle is a plasmid found in most strains of the yeast Saccharomyces cerevisiae at approximately 60-100 copies per cell. The plasmid possesses the novel capacity for replicative amplification induced by site-specific recombination. To address the question of whether the recombination model is adequate to account for observed rates of 2-μm circle amplification, we developed a direct computational simulation of the amplification system. Results of this simulation show that theoretically at least six copies per plasmid can be produced in each generation, and that previously unanticipated replication intermediates contribute largely to this degree of amplification.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 369-385 |
| Number of pages | 17 |
| Journal | Journal of Theoretical Biology |
| Volume | 155 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 7 1992 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Applied Mathematics
- Modeling and Simulation
- Statistics and Probability
- Medicine(all)
Cite this
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Direct simulation of yeast 2-μm circle plasmid amplification. / Russo, Frank D.; Scherson, Isaac; Broach, James.
In: Journal of Theoretical Biology, Vol. 155, No. 3, 07.04.1992, p. 369-385.Research output: Contribution to journal › Article
TY - JOUR
T1 - Direct simulation of yeast 2-μm circle plasmid amplification
AU - Russo, Frank D.
AU - Scherson, Isaac
AU - Broach, James
PY - 1992/4/7
Y1 - 1992/4/7
N2 - The 2-μm circle is a plasmid found in most strains of the yeast Saccharomyces cerevisiae at approximately 60-100 copies per cell. The plasmid possesses the novel capacity for replicative amplification induced by site-specific recombination. To address the question of whether the recombination model is adequate to account for observed rates of 2-μm circle amplification, we developed a direct computational simulation of the amplification system. Results of this simulation show that theoretically at least six copies per plasmid can be produced in each generation, and that previously unanticipated replication intermediates contribute largely to this degree of amplification.
AB - The 2-μm circle is a plasmid found in most strains of the yeast Saccharomyces cerevisiae at approximately 60-100 copies per cell. The plasmid possesses the novel capacity for replicative amplification induced by site-specific recombination. To address the question of whether the recombination model is adequate to account for observed rates of 2-μm circle amplification, we developed a direct computational simulation of the amplification system. Results of this simulation show that theoretically at least six copies per plasmid can be produced in each generation, and that previously unanticipated replication intermediates contribute largely to this degree of amplification.
UR - http://www.scopus.com/inward/record.url?scp=0026594008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026594008&partnerID=8YFLogxK
U2 - 10.1016/S0022-5193(05)80604-6
DO - 10.1016/S0022-5193(05)80604-6
M3 - Article
C2 - 1619957
AN - SCOPUS:0026594008
VL - 155
SP - 369
EP - 385
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
SN - 0022-5193
IS - 3
ER -