TY - JOUR
T1 - Direct visualization and profiling of protein misfolding and aggregation in live cells
AU - Tang, Sicheng
AU - Wang, Wenting
AU - Zhang, Xin
N1 - Funding Information:
The authors thank for the support from the Burroughs Welcome Fund Career Award at the Scientific Interface 1013904 (X.Z.), Paul Berg Early Career Professorship (X.Z.), Lloyd and Dottie Huck Early Career Award (X.Z.), Sloan Research Fellowship FG-2018-10958 (X.Z.), PEW Biomedical Scholars Program 00033066 (X.Z.), and National Institute of Health R35 GM133484 (X.Z.).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10
Y1 - 2021/10
N2 - Over the past few years, research tools have been developed to monitor the multistep protein aggregation process in live cells, a process that has been associated with a growing number of human diseases. Herein, we describe recent advances in methods that can either survey the distribution of aggregation at the level of the cellular proteome using mass spectroscopy or discern the multistep aggregation process of specific proteins of interest via fluorescence signals. Future development and application of such technologies are expected to provide insights on mechanisms, diagnosis, and treatment of diseases rooted in protein aggregation.
AB - Over the past few years, research tools have been developed to monitor the multistep protein aggregation process in live cells, a process that has been associated with a growing number of human diseases. Herein, we describe recent advances in methods that can either survey the distribution of aggregation at the level of the cellular proteome using mass spectroscopy or discern the multistep aggregation process of specific proteins of interest via fluorescence signals. Future development and application of such technologies are expected to provide insights on mechanisms, diagnosis, and treatment of diseases rooted in protein aggregation.
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U2 - 10.1016/j.cbpa.2021.05.008
DO - 10.1016/j.cbpa.2021.05.008
M3 - Review article
C2 - 34246835
AN - SCOPUS:85109451790
VL - 64
SP - 116
EP - 123
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
ER -