Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

Xiaojun Lian, Jianhua Zhang, Samira M. Azarin, Kexian Zhu, Laurie B. Hazeltine, Xiaoping Bao, Cheston Hsiao, Timothy J. Kamp, Sean P. Palecek

Research output: Contribution to journalArticle

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Abstract

The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to functional cardiomyocytes in a completely defined, growth factor- and serum-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate temporal application of a glycogen synthase kinase 3 (GSK3) inhibitor combined with the expression of β-catenin shRNA or a chemical Wnt inhibitor is sufficient to produce a high yield (0.8-1.3 million cardiomyocytes per cm 2) of virtually pure (80-98%) functional cardiomyocytes in 14 d from multiple hPSC lines without cell sorting or selection. Qualitative (immunostaining) and quantitative (flow cytometry) characterization of differentiated cells is described to assess the expression of cardiac transcription factors and myofilament proteins. Flow cytometry of BrdU incorporation or Ki67 expression in conjunction with cardiac sarcomere myosin protein expression can be used to determine the proliferative capacity of hPSC-derived cardiomyocytes. Functional human cardiomyocytes differentiated via these protocols may constitute a potential cell source for heart disease modeling, drug screening and cell-based therapeutic applications.

Original languageEnglish (US)
Pages (from-to)162-175
Number of pages14
JournalNature Protocols
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2013

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Catenins
Pluripotent Stem Cells
Stem cells
Cardiac Myocytes
Flow cytometry
Cardiac Myosins
Glycogen Synthase Kinase 3
Bromodeoxyuridine
Flow Cytometry
Sorting
Small Interfering RNA
Intercellular Signaling Peptides and Proteins
Screening
Proteins
Transcription Factors
Sarcomeres
Cells
Preclinical Drug Evaluations
Modulation
Myofibrils

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lian, Xiaojun ; Zhang, Jianhua ; Azarin, Samira M. ; Zhu, Kexian ; Hazeltine, Laurie B. ; Bao, Xiaoping ; Hsiao, Cheston ; Kamp, Timothy J. ; Palecek, Sean P. / Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. In: Nature Protocols. 2013 ; Vol. 8, No. 1. pp. 162-175.
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Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. / Lian, Xiaojun; Zhang, Jianhua; Azarin, Samira M.; Zhu, Kexian; Hazeltine, Laurie B.; Bao, Xiaoping; Hsiao, Cheston; Kamp, Timothy J.; Palecek, Sean P.

In: Nature Protocols, Vol. 8, No. 1, 01.01.2013, p. 162-175.

Research output: Contribution to journalArticle

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