Directed endothelial progenitor differentiation from human pluripotent stem cells via wnt activation under defined conditions

Xiaoping Bao, Xiaojun Lian, Sean P. Palecek

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Efficient derivation of endothelial cells and their progenitors from human pluripotent stem cells (hPSCs) can facilitate studies of human vascular development, disease modeling, drug discovery, and cell-based therapy. Here we provide a detailed protocol for directing hPSCs to functional endothelial cells and their progenitors in a completely defined, growth factor- and serum-free system by temporal modulation of Wnt/β-catenin signaling via small molecules. We demonstrate a 10-day, two-stage process that recapitulates endothelial cell development, in which hPSCs first differentiate to endothelial progenitors that then generate functional endothelial cells and smooth muscle cells. Methods to characterize endothelial cell identity and function are also described.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages183-196
Number of pages14
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1481
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Bao, X., Lian, X., & Palecek, S. P. (2016). Directed endothelial progenitor differentiation from human pluripotent stem cells via wnt activation under defined conditions. In Methods in Molecular Biology (pp. 183-196). (Methods in Molecular Biology; Vol. 1481). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6393-5_17