Directing transport of CoFe2O4-functionalized microtubules with magnetic fields

Benjamin M. Hutchins, Mark Platt, William O. Hancock, Mary Elizabeth Williams

Research output: Contribution to journalReview article

61 Citations (Scopus)

Abstract

The external magnetic fields can be used to control the transport direction of magnetically labeled microtubules driven by kinesin motors in vitro without affecting their transport speed by selectively functionalizing microtubule segments with 20-nm CoFe2O4 magnetic nanoparticles. Kinesin motor proteins convert chemical energy derived from the hydrolysis of adenosine triphosphate (ATP) into mechanical energy to move intracellular cargo along microtubules, cylindrical polymers of the tubulin that act as intracellular conduits. This approach provides a significant alternative to serial methods such as optical tweezers. Magnetically labeled microtubules additionally provide a new tool for in vitro investigations of the role of microtubules and motors in important cellular processes such as cell division, axonal transport, and flagellar motility.

Original languageEnglish (US)
Pages (from-to)126-131
Number of pages6
JournalSmall
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Magnetic Fields
Microtubules
Kinesin
Magnetic fields
Optical tweezers
Tubulin
Optical Tweezers
Hydrolysis
Polymers
Adenosine Triphosphate
Cells
Axonal Transport
Nanoparticles
Proteins
Cell Division
cobalt ferrite
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

@article{59a30889d7e244a9b04108ac65234de3,
title = "Directing transport of CoFe2O4-functionalized microtubules with magnetic fields",
abstract = "The external magnetic fields can be used to control the transport direction of magnetically labeled microtubules driven by kinesin motors in vitro without affecting their transport speed by selectively functionalizing microtubule segments with 20-nm CoFe2O4 magnetic nanoparticles. Kinesin motor proteins convert chemical energy derived from the hydrolysis of adenosine triphosphate (ATP) into mechanical energy to move intracellular cargo along microtubules, cylindrical polymers of the tubulin that act as intracellular conduits. This approach provides a significant alternative to serial methods such as optical tweezers. Magnetically labeled microtubules additionally provide a new tool for in vitro investigations of the role of microtubules and motors in important cellular processes such as cell division, axonal transport, and flagellar motility.",
author = "Hutchins, {Benjamin M.} and Mark Platt and Hancock, {William O.} and Williams, {Mary Elizabeth}",
year = "2007",
month = "1",
day = "1",
doi = "10.1002/smll.200600410",
language = "English (US)",
volume = "3",
pages = "126--131",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "1",

}

Directing transport of CoFe2O4-functionalized microtubules with magnetic fields. / Hutchins, Benjamin M.; Platt, Mark; Hancock, William O.; Williams, Mary Elizabeth.

In: Small, Vol. 3, No. 1, 01.01.2007, p. 126-131.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Directing transport of CoFe2O4-functionalized microtubules with magnetic fields

AU - Hutchins, Benjamin M.

AU - Platt, Mark

AU - Hancock, William O.

AU - Williams, Mary Elizabeth

PY - 2007/1/1

Y1 - 2007/1/1

N2 - The external magnetic fields can be used to control the transport direction of magnetically labeled microtubules driven by kinesin motors in vitro without affecting their transport speed by selectively functionalizing microtubule segments with 20-nm CoFe2O4 magnetic nanoparticles. Kinesin motor proteins convert chemical energy derived from the hydrolysis of adenosine triphosphate (ATP) into mechanical energy to move intracellular cargo along microtubules, cylindrical polymers of the tubulin that act as intracellular conduits. This approach provides a significant alternative to serial methods such as optical tweezers. Magnetically labeled microtubules additionally provide a new tool for in vitro investigations of the role of microtubules and motors in important cellular processes such as cell division, axonal transport, and flagellar motility.

AB - The external magnetic fields can be used to control the transport direction of magnetically labeled microtubules driven by kinesin motors in vitro without affecting their transport speed by selectively functionalizing microtubule segments with 20-nm CoFe2O4 magnetic nanoparticles. Kinesin motor proteins convert chemical energy derived from the hydrolysis of adenosine triphosphate (ATP) into mechanical energy to move intracellular cargo along microtubules, cylindrical polymers of the tubulin that act as intracellular conduits. This approach provides a significant alternative to serial methods such as optical tweezers. Magnetically labeled microtubules additionally provide a new tool for in vitro investigations of the role of microtubules and motors in important cellular processes such as cell division, axonal transport, and flagellar motility.

UR - http://www.scopus.com/inward/record.url?scp=33846325180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846325180&partnerID=8YFLogxK

U2 - 10.1002/smll.200600410

DO - 10.1002/smll.200600410

M3 - Review article

VL - 3

SP - 126

EP - 131

JO - Small

JF - Small

SN - 1613-6810

IS - 1

ER -