Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture

Peter Simon, Peter Houston, James Broach

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intra-chromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type inter-conversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands.

Original languageEnglish (US)
Pages (from-to)2282-2291
Number of pages10
JournalEMBO Journal
Volume21
Issue number9
DOIs
StatePublished - May 1 2002

Fingerprint

Saccharomyces
Chromosomes
Gene Conversion
Green Fluorescent Proteins
Haploidy
Genetic Recombination
DNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

@article{4fd6bb88c2514f189970a27e92be7db0,
title = "Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture",
abstract = "Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intra-chromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type inter-conversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands.",
author = "Peter Simon and Peter Houston and James Broach",
year = "2002",
month = "5",
day = "1",
doi = "10.1093/emboj/21.9.2282",
language = "English (US)",
volume = "21",
pages = "2282--2291",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "9",

}

Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture. / Simon, Peter; Houston, Peter; Broach, James.

In: EMBO Journal, Vol. 21, No. 9, 01.05.2002, p. 2282-2291.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture

AU - Simon, Peter

AU - Houston, Peter

AU - Broach, James

PY - 2002/5/1

Y1 - 2002/5/1

N2 - Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intra-chromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type inter-conversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands.

AB - Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intra-chromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type inter-conversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands.

UR - http://www.scopus.com/inward/record.url?scp=0036566327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036566327&partnerID=8YFLogxK

U2 - 10.1093/emboj/21.9.2282

DO - 10.1093/emboj/21.9.2282

M3 - Article

C2 - 11980725

AN - SCOPUS:0036566327

VL - 21

SP - 2282

EP - 2291

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 9

ER -