Discovery and complete genome sequence of a novel circovirus-like virus in the endangered rowi kiwi, Apteryx rowi

Daniel J. White, Richard J. Hall, Jing Wang, Nicole Moore, Duckchul Park, Kate McInnes, Brett D. Gartrell, Daniel M. Tompkins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Circoviruses are circular, non-enveloped, single-stranded DNA viruses around 2000 nucleotides (nt) in length and include the pathogenic species, Porcine circovirus 1 and Beak and feather disease virus, capable of causing significant morbidity and mortality. This group of viruses may be robust to degradation by external environments, and avian circoviruses are known to move between closely related hosts. Using a de novo metagenomic approach, followed by confirmatory PCR, we identify for the first time a circular Rep-encoding single-stranded (CRESS) DNA virus in New Zealand kiwi, Apteryx spp., derived from faecal matter of the rowi kiwi (A. rowi) showing signs of verminous dermatitis. The entire 2085 nt genome was cloned and sequenced and contains both capsid and replicase genes, as well as a conserved 9 nt motif. Phylogenetic analyses place it within Circoviridae, adjacent to other environmental CRESS-DNA viruses, and most closely related to badger circovirus-like virus (Meles meles circovirus-like virus). As the rowi is the most critically endangered kiwi, it is vital to understand the role of rowi kiwi circovirus-like virus as a possible pathogen and also any potential cross-species transmission.

Original languageEnglish (US)
Pages (from-to)727-731
Number of pages5
JournalVirus Genes
Volume52
Issue number5
DOIs
StatePublished - Oct 1 2016

Fingerprint

Circovirus
Genome
Viruses
DNA Viruses
Single-Stranded DNA
Circoviridae
Nucleotides
Mustelidae
Metagenomics
Nucleotide Motifs
Capsid
Dermatitis
New Zealand
Morbidity
Polymerase Chain Reaction
Mortality

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology

Cite this

White, Daniel J. ; Hall, Richard J. ; Wang, Jing ; Moore, Nicole ; Park, Duckchul ; McInnes, Kate ; Gartrell, Brett D. ; Tompkins, Daniel M. / Discovery and complete genome sequence of a novel circovirus-like virus in the endangered rowi kiwi, Apteryx rowi. In: Virus Genes. 2016 ; Vol. 52, No. 5. pp. 727-731.
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White, DJ, Hall, RJ, Wang, J, Moore, N, Park, D, McInnes, K, Gartrell, BD & Tompkins, DM 2016, 'Discovery and complete genome sequence of a novel circovirus-like virus in the endangered rowi kiwi, Apteryx rowi', Virus Genes, vol. 52, no. 5, pp. 727-731. https://doi.org/10.1007/s11262-016-1342-x

Discovery and complete genome sequence of a novel circovirus-like virus in the endangered rowi kiwi, Apteryx rowi. / White, Daniel J.; Hall, Richard J.; Wang, Jing; Moore, Nicole; Park, Duckchul; McInnes, Kate; Gartrell, Brett D.; Tompkins, Daniel M.

In: Virus Genes, Vol. 52, No. 5, 01.10.2016, p. 727-731.

Research output: Contribution to journalArticle

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