Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris

Venkata G. Poosarla, Thammajun Leungsakul Wood, Lei Zhu, Daniel S. Miller, Bei Yin, Thomas Keith Wood

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion. The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein. In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating. Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms. In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms. Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.

Original languageEnglish (US)
Pages (from-to)779-787
Number of pages9
JournalEnvironmental Microbiology Reports
Volume9
Issue number6
DOIs
StatePublished - Dec 1 2017

Fingerprint

Desulfovibrio vulgaris
Mannose
Biofilms
mannose
biofilm
glucose
Glucose
sulfate-reducing bacteria
sulfate-reducing bacterium
Sulfates
Bacteria
Acetylgalactosamine
Fucose
Corrosion
fucose
corrosion
Lectins
lectins
control methods
enzyme

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Poosarla, Venkata G. ; Wood, Thammajun Leungsakul ; Zhu, Lei ; Miller, Daniel S. ; Yin, Bei ; Wood, Thomas Keith. / Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris. In: Environmental Microbiology Reports. 2017 ; Vol. 9, No. 6. pp. 779-787.
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Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris. / Poosarla, Venkata G.; Wood, Thammajun Leungsakul; Zhu, Lei; Miller, Daniel S.; Yin, Bei; Wood, Thomas Keith.

In: Environmental Microbiology Reports, Vol. 9, No. 6, 01.12.2017, p. 779-787.

Research output: Contribution to journalArticle

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AU - Zhu, Lei

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