Displacement and hybridization reactions in aptamer-functionalized hydrogels for biomimetic protein release and signal transduction

Jinping Lai, Shihui Li, Xuechen Shi, James Coyne, Nan Zhao, Fengping Dong, Yingwei Mao, Yong Wang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A variety of hydrogels have been synthesized for controlling the release of signaling molecules in applications such as drug delivery and regenerative medicine. However, it remains challenging to synthesize hydrogels with the ability to control the release of signaling molecules sequentially or periodically under physiological conditions as living cells do in response to the variation of metabolism. The purpose of this work was to study a novel biomimetic hydrogel system with the ability of recapitulating the procedure of cellular signal transduction and controlling the sequential release of signaling molecules under physiological conditions. In the presence of a small chemical, the signaling molecule is regulated to change from a DNA-bound state to a free state and the freed signaling molecule is able to regulate intracellular signal transduction and cell migration. Moreover, periodic exposure of the hydrogel system to the small chemical leads to sequential protein release. Since signaling molecules are important for every activity of the cell, this hydrogel system holds potential as a metabolism-responsive platform for controlled release of signaling molecules and cell regulation in various applications.

Original languageEnglish (US)
Pages (from-to)7306-7311
Number of pages6
JournalChemical Science
Volume8
Issue number11
DOIs
StatePublished - Jan 1 2017

Fingerprint

Signal transduction
Hydrogels
Biomimetics
Molecules
Hydrogel
Proteins
Metabolism
Cells
Drug delivery
DNA

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

@article{ed01d59cf6ab43cd805bfbe7c57aa93b,
title = "Displacement and hybridization reactions in aptamer-functionalized hydrogels for biomimetic protein release and signal transduction",
abstract = "A variety of hydrogels have been synthesized for controlling the release of signaling molecules in applications such as drug delivery and regenerative medicine. However, it remains challenging to synthesize hydrogels with the ability to control the release of signaling molecules sequentially or periodically under physiological conditions as living cells do in response to the variation of metabolism. The purpose of this work was to study a novel biomimetic hydrogel system with the ability of recapitulating the procedure of cellular signal transduction and controlling the sequential release of signaling molecules under physiological conditions. In the presence of a small chemical, the signaling molecule is regulated to change from a DNA-bound state to a free state and the freed signaling molecule is able to regulate intracellular signal transduction and cell migration. Moreover, periodic exposure of the hydrogel system to the small chemical leads to sequential protein release. Since signaling molecules are important for every activity of the cell, this hydrogel system holds potential as a metabolism-responsive platform for controlled release of signaling molecules and cell regulation in various applications.",
author = "Jinping Lai and Shihui Li and Xuechen Shi and James Coyne and Nan Zhao and Fengping Dong and Yingwei Mao and Yong Wang",
year = "2017",
month = "1",
day = "1",
doi = "10.1039/c7sc03023a",
language = "English (US)",
volume = "8",
pages = "7306--7311",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "11",

}

Displacement and hybridization reactions in aptamer-functionalized hydrogels for biomimetic protein release and signal transduction. / Lai, Jinping; Li, Shihui; Shi, Xuechen; Coyne, James; Zhao, Nan; Dong, Fengping; Mao, Yingwei; Wang, Yong.

In: Chemical Science, Vol. 8, No. 11, 01.01.2017, p. 7306-7311.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Displacement and hybridization reactions in aptamer-functionalized hydrogels for biomimetic protein release and signal transduction

AU - Lai, Jinping

AU - Li, Shihui

AU - Shi, Xuechen

AU - Coyne, James

AU - Zhao, Nan

AU - Dong, Fengping

AU - Mao, Yingwei

AU - Wang, Yong

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A variety of hydrogels have been synthesized for controlling the release of signaling molecules in applications such as drug delivery and regenerative medicine. However, it remains challenging to synthesize hydrogels with the ability to control the release of signaling molecules sequentially or periodically under physiological conditions as living cells do in response to the variation of metabolism. The purpose of this work was to study a novel biomimetic hydrogel system with the ability of recapitulating the procedure of cellular signal transduction and controlling the sequential release of signaling molecules under physiological conditions. In the presence of a small chemical, the signaling molecule is regulated to change from a DNA-bound state to a free state and the freed signaling molecule is able to regulate intracellular signal transduction and cell migration. Moreover, periodic exposure of the hydrogel system to the small chemical leads to sequential protein release. Since signaling molecules are important for every activity of the cell, this hydrogel system holds potential as a metabolism-responsive platform for controlled release of signaling molecules and cell regulation in various applications.

AB - A variety of hydrogels have been synthesized for controlling the release of signaling molecules in applications such as drug delivery and regenerative medicine. However, it remains challenging to synthesize hydrogels with the ability to control the release of signaling molecules sequentially or periodically under physiological conditions as living cells do in response to the variation of metabolism. The purpose of this work was to study a novel biomimetic hydrogel system with the ability of recapitulating the procedure of cellular signal transduction and controlling the sequential release of signaling molecules under physiological conditions. In the presence of a small chemical, the signaling molecule is regulated to change from a DNA-bound state to a free state and the freed signaling molecule is able to regulate intracellular signal transduction and cell migration. Moreover, periodic exposure of the hydrogel system to the small chemical leads to sequential protein release. Since signaling molecules are important for every activity of the cell, this hydrogel system holds potential as a metabolism-responsive platform for controlled release of signaling molecules and cell regulation in various applications.

UR - http://www.scopus.com/inward/record.url?scp=85032037572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032037572&partnerID=8YFLogxK

U2 - 10.1039/c7sc03023a

DO - 10.1039/c7sc03023a

M3 - Article

C2 - 29163881

AN - SCOPUS:85032037572

VL - 8

SP - 7306

EP - 7311

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 11

ER -