Dissecting resistance to phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and qtl mapping

Tetyana N. Zhebentyayeva, Paul H. Sisco, Laura L. Georgi, Steven N. Jeffers, M. Taylor Perkins, Joseph B. James, Frederick V. Hebard, Christopher Saski, C. Dana Nelson, Albert G. Abbott

Research output: Contribution to journalArticle

Abstract

The soilborne oomycete Phytophthora cinnamomi-which causes root rot, trunk cankers, and stem lesions on an estimated 5,000 plant species worldwide-is a lethal pathogen of American chestnut (Castanea dentata) as well as many other woody plant species. P. cinnamomi is particularly damaging to chestnut and chinquapin trees (Castanea spp.) in the southern portion of its native range in the United States due to relatively mild climatic conditions that are conductive to disease development. Introduction of resistant genotypes is the most practical solution for disease management in forests because treatment with fungicides and eradication of the pathogen are neither practical nor economically feasible in natural ecosystems. Using backcross families derived from crosses of American chestnuts with two resistant Chinese chestnut cultivars Mahogany and Nanking, we constructed linkage maps and identified quantitative trait loci (QTLs) for resistance to P. cinnamomi that had been introgressed from these Chinese chestnut cultivars. In total, 957 plants representing five cohorts of three hybrid crosses were genotyped by sequencing and phenotyped by standardized inoculation and visual examination over a 6-year period from 2011 to 2016. Eight parental linkage maps comprising 7,715 markers were constructed, and 17 QTLs were identified on four linkage groups (LGs): LG-A, LG-C, LG-E, and LG-K. The most consistent QTLs were detected on LG-E in seedlings from crosses with both 'Mahogany' and 'Nanking' and LG-K in seedlings from 'Mahogany' crosses. Two consistent large and medium effect QTLs located ;10 cM apart were present in the middle and at the lower end of LG-E; other QTLs were considered to have small effects. These results imply that the genetic architecture of resistance to P. cinnamomi in Chinese chestnut × American chestnut hybrid progeny may resemble the P. sojae-soybean pathosystem, with a few dominant QTLs along with quantitatively inherited partial resistance conferred by multiple small-effect QTLs.

Original languageEnglish (US)
Pages (from-to)1594-1604
Number of pages11
JournalPHYTOPATHOLOGY
Volume109
Issue number9
DOIs
StatePublished - Jan 1 2019

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Phytophthora cinnamomi
Castanea
linkage groups
genotyping
quantitative trait loci
Castanea dentata
Castanea mollissima
Meliaceae
chromosome mapping
cankers (plants)
lesions (plant)
seedlings
pathogens
Oomycetes
cultivars
root rot
lethal genes
woody plants
tree trunk
fungicides

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Zhebentyayeva, Tetyana N. ; Sisco, Paul H. ; Georgi, Laura L. ; Jeffers, Steven N. ; Perkins, M. Taylor ; James, Joseph B. ; Hebard, Frederick V. ; Saski, Christopher ; Nelson, C. Dana ; Abbott, Albert G. / Dissecting resistance to phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and qtl mapping. In: PHYTOPATHOLOGY. 2019 ; Vol. 109, No. 9. pp. 1594-1604.
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abstract = "The soilborne oomycete Phytophthora cinnamomi-which causes root rot, trunk cankers, and stem lesions on an estimated 5,000 plant species worldwide-is a lethal pathogen of American chestnut (Castanea dentata) as well as many other woody plant species. P. cinnamomi is particularly damaging to chestnut and chinquapin trees (Castanea spp.) in the southern portion of its native range in the United States due to relatively mild climatic conditions that are conductive to disease development. Introduction of resistant genotypes is the most practical solution for disease management in forests because treatment with fungicides and eradication of the pathogen are neither practical nor economically feasible in natural ecosystems. Using backcross families derived from crosses of American chestnuts with two resistant Chinese chestnut cultivars Mahogany and Nanking, we constructed linkage maps and identified quantitative trait loci (QTLs) for resistance to P. cinnamomi that had been introgressed from these Chinese chestnut cultivars. In total, 957 plants representing five cohorts of three hybrid crosses were genotyped by sequencing and phenotyped by standardized inoculation and visual examination over a 6-year period from 2011 to 2016. Eight parental linkage maps comprising 7,715 markers were constructed, and 17 QTLs were identified on four linkage groups (LGs): LG-A, LG-C, LG-E, and LG-K. The most consistent QTLs were detected on LG-E in seedlings from crosses with both 'Mahogany' and 'Nanking' and LG-K in seedlings from 'Mahogany' crosses. Two consistent large and medium effect QTLs located ;10 cM apart were present in the middle and at the lower end of LG-E; other QTLs were considered to have small effects. These results imply that the genetic architecture of resistance to P. cinnamomi in Chinese chestnut × American chestnut hybrid progeny may resemble the P. sojae-soybean pathosystem, with a few dominant QTLs along with quantitatively inherited partial resistance conferred by multiple small-effect QTLs.",
author = "Zhebentyayeva, {Tetyana N.} and Sisco, {Paul H.} and Georgi, {Laura L.} and Jeffers, {Steven N.} and Perkins, {M. Taylor} and James, {Joseph B.} and Hebard, {Frederick V.} and Christopher Saski and Nelson, {C. Dana} and Abbott, {Albert G.}",
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Zhebentyayeva, TN, Sisco, PH, Georgi, LL, Jeffers, SN, Perkins, MT, James, JB, Hebard, FV, Saski, C, Nelson, CD & Abbott, AG 2019, 'Dissecting resistance to phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and qtl mapping', PHYTOPATHOLOGY, vol. 109, no. 9, pp. 1594-1604. https://doi.org/10.1094/PHYTO-11-18-0425-R

Dissecting resistance to phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and qtl mapping. / Zhebentyayeva, Tetyana N.; Sisco, Paul H.; Georgi, Laura L.; Jeffers, Steven N.; Perkins, M. Taylor; James, Joseph B.; Hebard, Frederick V.; Saski, Christopher; Nelson, C. Dana; Abbott, Albert G.

In: PHYTOPATHOLOGY, Vol. 109, No. 9, 01.01.2019, p. 1594-1604.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dissecting resistance to phytophthora cinnamomi in interspecific hybrid chestnut crosses using sequence-based genotyping and qtl mapping

AU - Zhebentyayeva, Tetyana N.

AU - Sisco, Paul H.

AU - Georgi, Laura L.

AU - Jeffers, Steven N.

AU - Perkins, M. Taylor

AU - James, Joseph B.

AU - Hebard, Frederick V.

AU - Saski, Christopher

AU - Nelson, C. Dana

AU - Abbott, Albert G.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The soilborne oomycete Phytophthora cinnamomi-which causes root rot, trunk cankers, and stem lesions on an estimated 5,000 plant species worldwide-is a lethal pathogen of American chestnut (Castanea dentata) as well as many other woody plant species. P. cinnamomi is particularly damaging to chestnut and chinquapin trees (Castanea spp.) in the southern portion of its native range in the United States due to relatively mild climatic conditions that are conductive to disease development. Introduction of resistant genotypes is the most practical solution for disease management in forests because treatment with fungicides and eradication of the pathogen are neither practical nor economically feasible in natural ecosystems. Using backcross families derived from crosses of American chestnuts with two resistant Chinese chestnut cultivars Mahogany and Nanking, we constructed linkage maps and identified quantitative trait loci (QTLs) for resistance to P. cinnamomi that had been introgressed from these Chinese chestnut cultivars. In total, 957 plants representing five cohorts of three hybrid crosses were genotyped by sequencing and phenotyped by standardized inoculation and visual examination over a 6-year period from 2011 to 2016. Eight parental linkage maps comprising 7,715 markers were constructed, and 17 QTLs were identified on four linkage groups (LGs): LG-A, LG-C, LG-E, and LG-K. The most consistent QTLs were detected on LG-E in seedlings from crosses with both 'Mahogany' and 'Nanking' and LG-K in seedlings from 'Mahogany' crosses. Two consistent large and medium effect QTLs located ;10 cM apart were present in the middle and at the lower end of LG-E; other QTLs were considered to have small effects. These results imply that the genetic architecture of resistance to P. cinnamomi in Chinese chestnut × American chestnut hybrid progeny may resemble the P. sojae-soybean pathosystem, with a few dominant QTLs along with quantitatively inherited partial resistance conferred by multiple small-effect QTLs.

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