Dissection of an antibody-catalyzed reaction

Jon D. Stewart, Joseph F. Krebs, Gary Siuzdak, Anthony J. Berdis, David B. Smithrud, Stephen J. Benkovic

Research output: Contribution to journalReview article

48 Citations (Scopus)

Abstract

Antibody 43C9 accelerates the hydrolysis of a p-nitroanilide by a factor of 2.5 x 105 over the background rate in addition to catalyzing the hydrolysis of a series of aromatic esters. Since this represents one of the largest rate accelerations achieved with an antibody, we have undertaken a series of studies aimed at uncovering the catalytic mechanism of 43C9. The immunogen, a phosphonamidate, was designed to mimic the geometric and electronic characteristics of the tetrahedral intermediate that forms upon nucleophilic attack by hydroxide on the amide substrate. Further studies, however, revealed that the catalytic mechanism is more complex and involves the fortuitous formation of a covalent acyl-antibody intermediate as a consequence of complementary side chain residues at the antibody-binding site. Several lines of evidence indicate that the catalytic mechanism involves two key residues: His-L91, which acts as a nucleophile to form the acyl-antibody intermediate, and Arg-L96, which stabilizes the anionic tetrahedral moieties. Support for this mechanism derives from the results of site-directed mutagenesis experiments and solvent deuterium isotope effects as well as direct detection of the acyl-antibody by electrospray mass spectrometry. Despite its partial recapitulation of the course of action of enzymic counterparts, the reactivity of 43C9, like other antibodies, is apparently limited by its affinity for the inducing immunogen. To go beyond this level, one must introduce additional catalytic functionality, particularly general acid-base catalysis, through either improvements in transition-state analog design or site-specific mutagenesis.

Original languageEnglish (US)
Pages (from-to)7404-7409
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number16
DOIs
StatePublished - Aug 2 1994

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Dissection
Antibodies
Site-Directed Mutagenesis
Hydrolysis
Antibody Binding Sites
Deuterium
Catalysis
Amides
Isotopes
Mass Spectrometry
Esters
Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

Stewart, J. D., Krebs, J. F., Siuzdak, G., Berdis, A. J., Smithrud, D. B., & Benkovic, S. J. (1994). Dissection of an antibody-catalyzed reaction. Proceedings of the National Academy of Sciences of the United States of America, 91(16), 7404-7409. https://doi.org/10.1073/pnas.91.16.7404
Stewart, Jon D. ; Krebs, Joseph F. ; Siuzdak, Gary ; Berdis, Anthony J. ; Smithrud, David B. ; Benkovic, Stephen J. / Dissection of an antibody-catalyzed reaction. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 16. pp. 7404-7409.
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Stewart, JD, Krebs, JF, Siuzdak, G, Berdis, AJ, Smithrud, DB & Benkovic, SJ 1994, 'Dissection of an antibody-catalyzed reaction', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 16, pp. 7404-7409. https://doi.org/10.1073/pnas.91.16.7404

Dissection of an antibody-catalyzed reaction. / Stewart, Jon D.; Krebs, Joseph F.; Siuzdak, Gary; Berdis, Anthony J.; Smithrud, David B.; Benkovic, Stephen J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 16, 02.08.1994, p. 7404-7409.

Research output: Contribution to journalReview article

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T1 - Dissection of an antibody-catalyzed reaction

AU - Stewart, Jon D.

AU - Krebs, Joseph F.

AU - Siuzdak, Gary

AU - Berdis, Anthony J.

AU - Smithrud, David B.

AU - Benkovic, Stephen J.

PY - 1994/8/2

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N2 - Antibody 43C9 accelerates the hydrolysis of a p-nitroanilide by a factor of 2.5 x 105 over the background rate in addition to catalyzing the hydrolysis of a series of aromatic esters. Since this represents one of the largest rate accelerations achieved with an antibody, we have undertaken a series of studies aimed at uncovering the catalytic mechanism of 43C9. The immunogen, a phosphonamidate, was designed to mimic the geometric and electronic characteristics of the tetrahedral intermediate that forms upon nucleophilic attack by hydroxide on the amide substrate. Further studies, however, revealed that the catalytic mechanism is more complex and involves the fortuitous formation of a covalent acyl-antibody intermediate as a consequence of complementary side chain residues at the antibody-binding site. Several lines of evidence indicate that the catalytic mechanism involves two key residues: His-L91, which acts as a nucleophile to form the acyl-antibody intermediate, and Arg-L96, which stabilizes the anionic tetrahedral moieties. Support for this mechanism derives from the results of site-directed mutagenesis experiments and solvent deuterium isotope effects as well as direct detection of the acyl-antibody by electrospray mass spectrometry. Despite its partial recapitulation of the course of action of enzymic counterparts, the reactivity of 43C9, like other antibodies, is apparently limited by its affinity for the inducing immunogen. To go beyond this level, one must introduce additional catalytic functionality, particularly general acid-base catalysis, through either improvements in transition-state analog design or site-specific mutagenesis.

AB - Antibody 43C9 accelerates the hydrolysis of a p-nitroanilide by a factor of 2.5 x 105 over the background rate in addition to catalyzing the hydrolysis of a series of aromatic esters. Since this represents one of the largest rate accelerations achieved with an antibody, we have undertaken a series of studies aimed at uncovering the catalytic mechanism of 43C9. The immunogen, a phosphonamidate, was designed to mimic the geometric and electronic characteristics of the tetrahedral intermediate that forms upon nucleophilic attack by hydroxide on the amide substrate. Further studies, however, revealed that the catalytic mechanism is more complex and involves the fortuitous formation of a covalent acyl-antibody intermediate as a consequence of complementary side chain residues at the antibody-binding site. Several lines of evidence indicate that the catalytic mechanism involves two key residues: His-L91, which acts as a nucleophile to form the acyl-antibody intermediate, and Arg-L96, which stabilizes the anionic tetrahedral moieties. Support for this mechanism derives from the results of site-directed mutagenesis experiments and solvent deuterium isotope effects as well as direct detection of the acyl-antibody by electrospray mass spectrometry. Despite its partial recapitulation of the course of action of enzymic counterparts, the reactivity of 43C9, like other antibodies, is apparently limited by its affinity for the inducing immunogen. To go beyond this level, one must introduce additional catalytic functionality, particularly general acid-base catalysis, through either improvements in transition-state analog design or site-specific mutagenesis.

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