Distribution and density of substance P receptors in the feline gastrointestinal tract using autoradiography

Robin D. Rothstein, Eric Johnson, Ann Ouyang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Autoradiography was used to localize and quantify substance P receptors in the feline gastrointestinal tract. The specific binding of 125I-Bolton Hunter substance P was determined in the esophagus, lower esophageal sphincter, antrum, pylorus, duodenum, jejunum, ileum, ileocecal sphincter, and colon. Competitive binding studies indicated that substance P binding sites or NK-1 receptor sites were demonstrated. The concentration of NK-1 receptors was greatest in the distal half of the gastrointestinal tract, with the highest concentrations in the proximal colon. The circular muscle layer contained the greatest amount of substance P binding. The location and density of binding sites for substance P may be important in understanding the relative importance of both the pharmacological responses to this neuropeptide and the immunohistochemical evidence of the peptide at different sites in the intestine.

Original languageEnglish (US)
Pages (from-to)1576-1581
Number of pages6
JournalGastroenterology
Volume100
Issue number6
DOIs
StatePublished - Jan 1 1991

Fingerprint

Neurokinin-1 Receptors
Felidae
Substance P
Autoradiography
Gastrointestinal Tract
Colon
Binding Sites
Lower Esophageal Sphincter
Competitive Binding
Pylorus
Jejunum
Neuropeptides
Ileum
Duodenum
Esophagus
Intestines
Pharmacology
Muscles
Peptides

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

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abstract = "Autoradiography was used to localize and quantify substance P receptors in the feline gastrointestinal tract. The specific binding of 125I-Bolton Hunter substance P was determined in the esophagus, lower esophageal sphincter, antrum, pylorus, duodenum, jejunum, ileum, ileocecal sphincter, and colon. Competitive binding studies indicated that substance P binding sites or NK-1 receptor sites were demonstrated. The concentration of NK-1 receptors was greatest in the distal half of the gastrointestinal tract, with the highest concentrations in the proximal colon. The circular muscle layer contained the greatest amount of substance P binding. The location and density of binding sites for substance P may be important in understanding the relative importance of both the pharmacological responses to this neuropeptide and the immunohistochemical evidence of the peptide at different sites in the intestine.",
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Distribution and density of substance P receptors in the feline gastrointestinal tract using autoradiography. / Rothstein, Robin D.; Johnson, Eric; Ouyang, Ann.

In: Gastroenterology, Vol. 100, No. 6, 01.01.1991, p. 1576-1581.

Research output: Contribution to journalArticle

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