Distribution and expression of SNAP-25 immunoreactivity in rat brain, rat PC-12 cells and human SMS-KCNR neuroblastoma cells

George A. Oyler, J. W. Polli, G. A. Higgins, M. C. Wilson, M. L. Billingsley

Research output: Contribution to journalArticle

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Abstract

Immunocytochemical, immunoblotting and in situ hybridization studies were used to map the distribution of SNAP-25 protein and mRNA in the rodent nervous system. These experiments demonstrated that subsets of neurons expressed SNAP-25, and that several patterns of expression emerged: SNAP-25 expression in caudate nucleus was initially concentrated in axons, which subsequently was localized in presynaptic regions of these axons. Other regions, typified by neocortex, showed developmental increases and persistent adult neuronal immunoreactivity for SNAP-25. Finally, olfactory bulb contained neurons which initially expressed SNAP-25, but lost expression during maturation. Additional studies in cultured human and rat cell lines derived from neural crest suggested that SNAP-25 is expressed in such lines, but not in glial or fibroblast lines. Differentiation of rat PC-12 cells with nerve growth factor failed to alter steady-state levels of SNAP-25 protein; similar responses were seen in human SMS-KCNR neuroblastoma cells differentiated using retinoic acid. The presence of SNAP-25 in presynaptic regions of numerous neuronal subsets and in neural crest cell lines suggests that this protein subserves an important function in neuronal tissues.

Original languageEnglish (US)
Pages (from-to)133-146
Number of pages14
JournalDevelopmental Brain Research
Volume65
Issue number2
DOIs
StatePublished - Feb 21 1992

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Synaptosomal-Associated Protein 25
Neural Crest
Neuroblastoma
Axons
Neurons
Cell Line
Caudate Nucleus
Olfactory Bulb
Neocortex
Brain
Nerve Growth Factor
Tretinoin
Immunoblotting
Neuroglia
Nervous System
In Situ Hybridization
Rodentia
Fibroblasts
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

Cite this

Oyler, George A. ; Polli, J. W. ; Higgins, G. A. ; Wilson, M. C. ; Billingsley, M. L. / Distribution and expression of SNAP-25 immunoreactivity in rat brain, rat PC-12 cells and human SMS-KCNR neuroblastoma cells. In: Developmental Brain Research. 1992 ; Vol. 65, No. 2. pp. 133-146.
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Distribution and expression of SNAP-25 immunoreactivity in rat brain, rat PC-12 cells and human SMS-KCNR neuroblastoma cells. / Oyler, George A.; Polli, J. W.; Higgins, G. A.; Wilson, M. C.; Billingsley, M. L.

In: Developmental Brain Research, Vol. 65, No. 2, 21.02.1992, p. 133-146.

Research output: Contribution to journalArticle

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