Distribution of β-Adrenoceptor subtypes in gastrointestinal tract of nondiabetic and diabetic BB rats a longitudinal study

O. Yu, A. Ouyang

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15 Citations (Scopus)

Abstract

The effects of aging and diabetes on the distribution of β- adrenoceptor subtypes in the gut were investigated in the BB rat. [125I]Cyanopindolol binding to 10-μm sections was evaluated using film autoradiography. Cyanopindolol binding to β-, β1-, and β2-adrenoceptors was displaced by 1 μM propranolol, 50 nM ICI-89-406, and 100 nM ICI-118- 551, respectively. β-Adrenoceptor binding was highest in the circular muscle of proximal colon and lowest in the pylorus of 4- to 5-month-old rats. Aging (8- to 10-month-old vs. 4- to 5-month-old rats) was associated with increased β-adrenoceptor binding in the pylorus and reduced binding in the proximal colon. Diabetes had a time-dependent effect on the level of β-adrenoceptor binding. It was increased in the antral and pyloric stomach but longer periods of diabetes caused a reduction in β-adrenoceptor binding in the pylorus. Those in the intestine were reduced time-dependently and involved β1-or β2-adrenoceptors or both.

Original languageEnglish (US)
Pages (from-to)1146-1153
Number of pages8
JournalDigestive Diseases and Sciences
Volume42
Issue number6
DOIs
StatePublished - Aug 27 1997

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Inbred BB Rats
Adrenergic Receptors
Longitudinal Studies
Gastrointestinal Tract
Pylorus
Colon
Autoradiography
Propranolol
Intestines
Stomach
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

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title = "Distribution of β-Adrenoceptor subtypes in gastrointestinal tract of nondiabetic and diabetic BB rats a longitudinal study",
abstract = "The effects of aging and diabetes on the distribution of β- adrenoceptor subtypes in the gut were investigated in the BB rat. [125I]Cyanopindolol binding to 10-μm sections was evaluated using film autoradiography. Cyanopindolol binding to β-, β1-, and β2-adrenoceptors was displaced by 1 μM propranolol, 50 nM ICI-89-406, and 100 nM ICI-118- 551, respectively. β-Adrenoceptor binding was highest in the circular muscle of proximal colon and lowest in the pylorus of 4- to 5-month-old rats. Aging (8- to 10-month-old vs. 4- to 5-month-old rats) was associated with increased β-adrenoceptor binding in the pylorus and reduced binding in the proximal colon. Diabetes had a time-dependent effect on the level of β-adrenoceptor binding. It was increased in the antral and pyloric stomach but longer periods of diabetes caused a reduction in β-adrenoceptor binding in the pylorus. Those in the intestine were reduced time-dependently and involved β1-or β2-adrenoceptors or both.",
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AB - The effects of aging and diabetes on the distribution of β- adrenoceptor subtypes in the gut were investigated in the BB rat. [125I]Cyanopindolol binding to 10-μm sections was evaluated using film autoradiography. Cyanopindolol binding to β-, β1-, and β2-adrenoceptors was displaced by 1 μM propranolol, 50 nM ICI-89-406, and 100 nM ICI-118- 551, respectively. β-Adrenoceptor binding was highest in the circular muscle of proximal colon and lowest in the pylorus of 4- to 5-month-old rats. Aging (8- to 10-month-old vs. 4- to 5-month-old rats) was associated with increased β-adrenoceptor binding in the pylorus and reduced binding in the proximal colon. Diabetes had a time-dependent effect on the level of β-adrenoceptor binding. It was increased in the antral and pyloric stomach but longer periods of diabetes caused a reduction in β-adrenoceptor binding in the pylorus. Those in the intestine were reduced time-dependently and involved β1-or β2-adrenoceptors or both.

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