Distribution of Guinea Pig Herpeslike Virus in Brain and Other Tissues of Naturally and Experimentally Infected Animals

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.

Original languageEnglish (US)
Pages (from-to)428-431
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume152
Issue number3
DOIs
StatePublished - Jan 1 1976

Fingerprint

Viruses
Brain
Guinea Pigs
Animals
Tissue
Trigeminal Ganglion
Blood
Virus Diseases
Coculture Techniques
Fluorescence Microscopy
Cell culture
Electron microscopy
Suspensions
Electron Microscopy
Leukocytes
Contamination
Spleen
Cell Culture Techniques
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{42fdbf23259b481b80745cab3f1914bd,
title = "Distribution of Guinea Pig Herpeslike Virus in Brain and Other Tissues of Naturally and Experimentally Infected Animals",
abstract = "Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.",
author = "Richard Tenser",
year = "1976",
month = "1",
day = "1",
doi = "10.3181/00379727-152-39412",
language = "English (US)",
volume = "152",
pages = "428--431",
journal = "Experimental Biology and Medicine",
issn = "1535-3702",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Distribution of Guinea Pig Herpeslike Virus in Brain and Other Tissues of Naturally and Experimentally Infected Animals

AU - Tenser, Richard

PY - 1976/1/1

Y1 - 1976/1/1

N2 - Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.

AB - Distribution of GPHLV in the trigeminal ganglion, brain, and other tissues of guinea pigs was studied. Virus was found in suspensions of all tissue cells from naturally infected guinea pigs, as well as animals inoculated ip or ic, up to 15 months after initiation of infection. Evidence of virus infection was detected by cultivation of cells or by cocultivation of infected cells with susceptible cell cultures, but not by other methods including immunofluorescence and electron microscopy. Quantitative determinations of virus in the trigeminal ganglia and different parts of the brain showed infectivity titers to be similar in these tissues. Since GPHLV was also found in blood and in high titer in spleen, and in other tissues tested, isolation of virus from neural tissues may have been due to contamination with infected blood leukocytes.

UR - http://www.scopus.com/inward/record.url?scp=0017117665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017117665&partnerID=8YFLogxK

U2 - 10.3181/00379727-152-39412

DO - 10.3181/00379727-152-39412

M3 - Article

C2 - 181762

AN - SCOPUS:0017117665

VL - 152

SP - 428

EP - 431

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 1535-3702

IS - 3

ER -