Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde

Russell C. Scaduto

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The reaction of orthophthaldehyde with glutathione at pH 8.0 is commonly employed as a specific fluorometric assay for glutathione. However, this method has been reported to overestimate the glutathione concentration when compared with either enzymatic or HPLC-based methods. We have found that amino acids and other thiols interfere in the reaction of glutathione with orthophthaldehyde, causing erroneous estimates of glutathione concentration. The extent of this interference is described.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalAnalytical Biochemistry
Volume174
Issue number1
DOIs
StatePublished - Oct 1988

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Dithiothreitol and amino acids interfere with the fluorometric determination of glutathione with orthophthaldehyde'. Together they form a unique fingerprint.

Cite this