Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes

Sanjay Anand, Wayne C.H. Wang, Dennis R. Powell, Stacey A. Bolanowski, Jian Zhang, Christina Ledje, Aruna B. Pawashe, Chris T. Amemiya, Cooduvalli S. Shashikant

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity.

Original languageEnglish (US)
Pages (from-to)15666-15669
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number26
DOIs
StatePublished - Dec 23 2003

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Homeobox Genes
Mammals
Fishes
Reporter Genes
Gene Expression
Thoracic Vertebrae
Developmental Genes
Nucleic Acid Regulatory Sequences
Regulator Genes
Transgenic Mice
Vertebrates
Embryonic Structures
Nucleotides

All Science Journal Classification (ASJC) codes

  • General

Cite this

Anand, Sanjay ; Wang, Wayne C.H. ; Powell, Dennis R. ; Bolanowski, Stacey A. ; Zhang, Jian ; Ledje, Christina ; Pawashe, Aruna B. ; Amemiya, Chris T. ; Shashikant, Cooduvalli S. / Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 26. pp. 15666-15669.
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Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes. / Anand, Sanjay; Wang, Wayne C.H.; Powell, Dennis R.; Bolanowski, Stacey A.; Zhang, Jian; Ledje, Christina; Pawashe, Aruna B.; Amemiya, Chris T.; Shashikant, Cooduvalli S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 26, 23.12.2003, p. 15666-15669.

Research output: Contribution to journalArticle

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AU - Ledje, Christina

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