Diversity of Pathotypes and DNA Fingerprint Haplotypes in Populations of Magnaporthe grisea in Korea over Two Decades

Sook Young Park, Michael G. Milgroom, Seong Sook Han, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Using isolates collected over 2 decades, we determined the population structure and dynamics of the rice blast fungus, Magnaporthe grisea, in Korea at both the genotypic and phenotypic levels. Pathotype analysis on 6,315 isolates collected from 328 rice cultivars from 1981 to 2000 revealed the presence of a total of 91 pathotypes. Among these 91 pathotypes, nine dominated, comprising 76.5% of the isolates. The expected number of pathotypes (corrected for sample size) increased significantly during the course of this study. On average, six (ranging from 0 to 20) new commercial cultivars were introduced annually between 1981 and 1998. However, the overall cultivar diversity, estimated using the Shannon index, was low. Most of the new cultivars were not planted to a large area because the seven most common cultivars each year occupied over 70% of the rice-cultivated area. The frequencies of the nine dominant pathotypes from these seven cultivars were highly correlated with those from the entire set of cultivars. To understand genetic diversity within and between pathotypes. 176 isolates collected from 1984 to 1999 were randomly sampled and analyzed by DNA fingerprinting. High similarities were observed among isolates; overall similarities were greater than 63% in combined MGR586 and MAGGY DNA fingerprints. Unlike most other populations of M. grisea. DNA fingerprints showed no clear lineage structure. No groups were supported by bootstrap values greater than 10%. Furthermore, there was no significant correlation between DNA fingerprint similarities and pathotypes. Genetic similarity was significantly greater (P < 0.001) within years than between years, although the difference was small. Our data suggest that M. grisea populations in Korea have been mostly dominated by a single clonal lineage. We cannot conclude from these data that selection by the host population has been a major force in the evolution of M. grisea in Korea.

Original languageEnglish (US)
Pages (from-to)1378-1385
Number of pages8
JournalPHYTOPATHOLOGY
Volume93
Issue number11
DOIs
StatePublished - Jan 1 2003

Fingerprint

Magnaporthe grisea
pathotypes
DNA fingerprinting
Korean Peninsula
haplotypes
cultivars
rice
blast disease
population structure
population dynamics
genetic variation
fungi

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Park, Sook Young ; Milgroom, Michael G. ; Han, Seong Sook ; Kang, Seogchan ; Lee, Yong Hwan. / Diversity of Pathotypes and DNA Fingerprint Haplotypes in Populations of Magnaporthe grisea in Korea over Two Decades. In: PHYTOPATHOLOGY. 2003 ; Vol. 93, No. 11. pp. 1378-1385.
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title = "Diversity of Pathotypes and DNA Fingerprint Haplotypes in Populations of Magnaporthe grisea in Korea over Two Decades",
abstract = "Using isolates collected over 2 decades, we determined the population structure and dynamics of the rice blast fungus, Magnaporthe grisea, in Korea at both the genotypic and phenotypic levels. Pathotype analysis on 6,315 isolates collected from 328 rice cultivars from 1981 to 2000 revealed the presence of a total of 91 pathotypes. Among these 91 pathotypes, nine dominated, comprising 76.5{\%} of the isolates. The expected number of pathotypes (corrected for sample size) increased significantly during the course of this study. On average, six (ranging from 0 to 20) new commercial cultivars were introduced annually between 1981 and 1998. However, the overall cultivar diversity, estimated using the Shannon index, was low. Most of the new cultivars were not planted to a large area because the seven most common cultivars each year occupied over 70{\%} of the rice-cultivated area. The frequencies of the nine dominant pathotypes from these seven cultivars were highly correlated with those from the entire set of cultivars. To understand genetic diversity within and between pathotypes. 176 isolates collected from 1984 to 1999 were randomly sampled and analyzed by DNA fingerprinting. High similarities were observed among isolates; overall similarities were greater than 63{\%} in combined MGR586 and MAGGY DNA fingerprints. Unlike most other populations of M. grisea. DNA fingerprints showed no clear lineage structure. No groups were supported by bootstrap values greater than 10{\%}. Furthermore, there was no significant correlation between DNA fingerprint similarities and pathotypes. Genetic similarity was significantly greater (P < 0.001) within years than between years, although the difference was small. Our data suggest that M. grisea populations in Korea have been mostly dominated by a single clonal lineage. We cannot conclude from these data that selection by the host population has been a major force in the evolution of M. grisea in Korea.",
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Diversity of Pathotypes and DNA Fingerprint Haplotypes in Populations of Magnaporthe grisea in Korea over Two Decades. / Park, Sook Young; Milgroom, Michael G.; Han, Seong Sook; Kang, Seogchan; Lee, Yong Hwan.

In: PHYTOPATHOLOGY, Vol. 93, No. 11, 01.01.2003, p. 1378-1385.

Research output: Contribution to journalArticle

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