DNA barcoding reveals limited accuracy of identifications based on folk taxonomy

Hugo J. De Boer, Abderrahim Ouarghidi, Gary Martin, Abdelaziz Abbad, Anneleen Kool

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: The trade of plant roots as traditional medicine is an important source of income for many people around the world. Destructive harvesting practices threaten the existence of some plant species. Harvesters of medicinal roots identify the collected species according to their own folk taxonomies, but once the dried or powdered roots enter the chain of commercialization, accurate identification becomes more challenging. Methodology: A survey of morphological diversity among four root products traded in the medina of Marrakech was conducted. Fifty-one root samples were selected for molecular identification using DNA barcoding using three markers, trnH-psbA, rpoC1, and ITS. Sequences were searched using BLAST against a tailored reference database of Moroccan medicinal plants and their closest relatives submitted to NCBI GenBank. Principal Findings: Combining psbA-trnH, rpoC1, and ITS allowed the majority of the market samples to be identified to species level. Few of the species level barcoding identifications matched the scientific names given in the literature, including the most authoritative and widely cited pharmacopeia. Conclusions/Significance: The four root complexes selected from the medicinal plant products traded in Marrakech all comprise more than one species, but not those previously asserted. The findings have major implications for the monitoring of trade in endangered plant species as morphology-based species identifications alone may not be accurate. As a result, trade in certain species may be overestimated, whereas the commercialization of other species may not be recorded at all.

Original languageEnglish (US)
Article numbere84291
JournalPloS one
Volume9
Issue number1
DOIs
StatePublished - Jan 8 2014

Fingerprint

DNA barcoding
Taxonomies
Medicinal Plants
commercialization
taxonomy
Endangered Species
Plant Roots
Pharmacopoeias
medicinal plants
DNA
Nucleic Acid Databases
Traditional Medicine
Names
Harvesters
plant products
barcoding
harvesters
Databases
traditional medicine
endangered species

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

De Boer, Hugo J. ; Ouarghidi, Abderrahim ; Martin, Gary ; Abbad, Abdelaziz ; Kool, Anneleen. / DNA barcoding reveals limited accuracy of identifications based on folk taxonomy. In: PloS one. 2014 ; Vol. 9, No. 1.
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DNA barcoding reveals limited accuracy of identifications based on folk taxonomy. / De Boer, Hugo J.; Ouarghidi, Abderrahim; Martin, Gary; Abbad, Abdelaziz; Kool, Anneleen.

In: PloS one, Vol. 9, No. 1, e84291, 08.01.2014.

Research output: Contribution to journalArticle

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