DNA fingerprinting of Pasteurella multocida recovered from avian sources

Alongkorn Amonsin, James F.X. Wellehan, Ling Ling Li, Judy Laber, Vivek Kapur

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices of discrimination. Concordance analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness among isolates of P. multocida. In addition, the data provide evidence of host specificity of P. multocida clones. Overall, the results of our study indicate that the rep-PCR and AFLP techniques enable rapid fingerprinting of P. multocida isolates from multiple avian species and enhance the investigation of fowl cholera outbreaks.

Original languageEnglish (US)
Pages (from-to)3025-3031
Number of pages7
JournalJournal of clinical microbiology
Volume40
Issue number8
DOIs
StatePublished - Aug 1 2002

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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