Characterisation of populations of Phragmidium violaceum in Australia is necessary to assess the efficacy of this exotic rust fungus for the biological control of various genotypes of European blackberry (Rubus fruticosus L. aggregate). P. violaceum was introduced to Australia on at least three separate occasions: the first being an unauthorised introduction of an unknown strain or strains in the early 1980s, and the second and third being authorised releases of the biological control agent, strain F15, in 1991 and 1992. Using restriction fragment length polymorphisms (RFLP) in minisatellite DNA (M13 probe), 13 DNA phenotypes of P. violaceum were identified among 18 single-pustule isolates collected from various locations in mainland Australia between 1997 and 1999. The restriction-fragment patterns and DNA band-sharing indices of similarity suggest that so-called 'illegal' strains predominate in populations of P. violaceum and that isolate F15 did not become well established in Australia.
All Science Journal Classification (ASJC) codes
- Plant Science