DNA replication of chimeric JC virus-simian virus 40 genomes

Kevin J. Lynch, Sheryl Haggerty, Richard John Frisque

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The ubiquitous virus JCV is the etiologic agent of the human brain disease progressive multifocal leukoencephalopathy. Although infection usually occurs early in life and the virus can remain latent in human tissues, including brain, little Information is available regarding its replication. It is known that DNA replication of primate polyomaviruses is dependent upon the synthesis of T antigen and the subsequent interactions of this protein with cellular factors and the viral origin of replication. We constructed chimeric genomes between JCV and SV40, two genetically similar viruses with distinct biologies, in which segments of the T antigen coding region and the replication origin were exchanged. Because the engineering of these genomes created a defect in the structural protein VP1, their DNA replicating activities could be compared without the complication of secondary infection of adjacent cells and amplification of the replication signal. The ability of the JCV-SV40 hybrid T antigens to initiate replication from the two viral origins in primate cells was investigated. A region of the JCV T antigen that includes the DNA binding and zinc finger domains was found to be responsible for the failure of JCV T antigen to interact productively with the SV40 origin. In addition, the ability to replicate in monkey cells was limited to constructs expressing T antigens which contained the carboxy-terminal host range domain of SV40.

Original languageEnglish (US)
Article number71600
Pages (from-to)819-822
Number of pages4
JournalVirology
Volume204
Issue number2
DOIs
StatePublished - Nov 1 1994

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JC Virus
Simian virus 40
Viral Tumor Antigens
DNA Replication
Genome
Replication Origin
Viruses
Primates
Progressive Multifocal Leukoencephalopathy
Polyomavirus Transforming Antigens
Polyomavirus
Host Specificity
DNA
Zinc Fingers
Brain Diseases
Coinfection
Haplorhini
Proteins
Brain
Infection

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Lynch, K. J., Haggerty, S., & Frisque, R. J. (1994). DNA replication of chimeric JC virus-simian virus 40 genomes. Virology, 204(2), 819-822. [71600]. https://doi.org/10.1006/viro.1994.1600
Lynch, Kevin J. ; Haggerty, Sheryl ; Frisque, Richard John. / DNA replication of chimeric JC virus-simian virus 40 genomes. In: Virology. 1994 ; Vol. 204, No. 2. pp. 819-822.
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Lynch, KJ, Haggerty, S & Frisque, RJ 1994, 'DNA replication of chimeric JC virus-simian virus 40 genomes', Virology, vol. 204, no. 2, 71600, pp. 819-822. https://doi.org/10.1006/viro.1994.1600

DNA replication of chimeric JC virus-simian virus 40 genomes. / Lynch, Kevin J.; Haggerty, Sheryl; Frisque, Richard John.

In: Virology, Vol. 204, No. 2, 71600, 01.11.1994, p. 819-822.

Research output: Contribution to journalArticle

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Lynch KJ, Haggerty S, Frisque RJ. DNA replication of chimeric JC virus-simian virus 40 genomes. Virology. 1994 Nov 1;204(2):819-822. 71600. https://doi.org/10.1006/viro.1994.1600