DNA synthesis in adult rat anterior pituitary glands in organ culture

Andrea Mastro, W. C. Hymer, C. D. Therrien

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Anterior pituitary glands of adult male rats weighing 175-500 g were cultured for periods up to 13 h in Medium 199 containing 2 μc/ml of 3H-thymidine. Extraction of the nucleic acids from pituitary homogenates by conventional biochemical procedures indicated that the 3H-thymidine was incorporated into DNA. In addition, radioautographs showed that the newly synthesized DNA was nuclear and in pituitary parenchymal cells. Labeled mitotic figures were found, and labeled acidophils and basophils were also observed. These results indicate that pituitary cells committed to the synthesis of specific hormones can also synthesize DNA.

Original languageEnglish (US)
Pages (from-to)407-414
Number of pages8
JournalExperimental Cell Research
Volume54
Issue number3
DOIs
StatePublished - Mar 1969

All Science Journal Classification (ASJC) codes

  • Cell Biology

Fingerprint Dive into the research topics of 'DNA synthesis in adult rat anterior pituitary glands in organ culture'. Together they form a unique fingerprint.

Cite this