DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits

R. Han, N. M. Cladel, C. A. Reed, Xuwen Peng, L. R. Budgeon, M. Pickel, Neil Christensen

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.

Original languageEnglish (US)
Pages (from-to)9712-9716
Number of pages5
JournalJournal of Virology
Volume74
Issue number20
DOIs
StatePublished - Jan 1 2000

Fingerprint

papilloma
Papillomaviridae
Papilloma
skin (animal)
carcinoma
Vaccination
rabbits
vaccination
Rabbits
Carcinoma
Skin
Cottontail rabbit papillomavirus
DNA
Genes
Firearms
genes
lesions (animal)
Immunization
plasmids
immunization

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Han, R. ; Cladel, N. M. ; Reed, C. A. ; Peng, Xuwen ; Budgeon, L. R. ; Pickel, M. ; Christensen, Neil. / DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits. In: Journal of Virology. 2000 ; Vol. 74, No. 20. pp. 9712-9716.
@article{c92f070ed3e64cf1b16c82ca9061098c,
title = "DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits",
abstract = "Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.",
author = "R. Han and Cladel, {N. M.} and Reed, {C. A.} and Xuwen Peng and Budgeon, {L. R.} and M. Pickel and Neil Christensen",
year = "2000",
month = "1",
day = "1",
doi = "10.1128/JVI.74.20.9712-9716.2000",
language = "English (US)",
volume = "74",
pages = "9712--9716",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "20",

}

DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits. / Han, R.; Cladel, N. M.; Reed, C. A.; Peng, Xuwen; Budgeon, L. R.; Pickel, M.; Christensen, Neil.

In: Journal of Virology, Vol. 74, No. 20, 01.01.2000, p. 9712-9716.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits

AU - Han, R.

AU - Cladel, N. M.

AU - Reed, C. A.

AU - Peng, Xuwen

AU - Budgeon, L. R.

AU - Pickel, M.

AU - Christensen, Neil

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.

AB - Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.

UR - http://www.scopus.com/inward/record.url?scp=0033814225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033814225&partnerID=8YFLogxK

U2 - 10.1128/JVI.74.20.9712-9716.2000

DO - 10.1128/JVI.74.20.9712-9716.2000

M3 - Article

C2 - 11000243

AN - SCOPUS:0033814225

VL - 74

SP - 9712

EP - 9716

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 20

ER -