DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits

R. Han, N. M. Cladel, C. A. Reed, X. Peng, L. R. Budgeon, M. Pickel, N. D. Christensen

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Malignant progression is a life-threatening consequence of human papillomavirus-associated lesions. In this study, we tested the efficacy of papillomavirus early-gene-based vaccines for prevention of carcinoma development of papillomavirus-induced skin papillomas on rabbits. Rabbit skin papillomas were initiated by infection with cottontail rabbit papillomavirus (CRPV). The papillomas were allowed to grow for 3 months without any treatment intervention. Rabbits were then immunized by gene gun-mediated intracutaneous administration of four DNA plasmids encoding CRPV E1, E2, E6, and E7 genes, respectively. All eight control rabbits receiving vector alone developed invasive carcinoma within 8 to 13 months. In contrast, only two of eight vaccinated rabbits developed carcinoma at 12 and 15 months, respectively. Papilloma growth was suppressed in the majority of vaccinated rabbits but not completely eradicated. These results indicate that gene gun-mediated immunization with papillomavirus early genes may be a promising strategy for prevention of malignant progression of human papillomavirus-associated lesions in humans.

Original languageEnglish (US)
Pages (from-to)9712-9716
Number of pages5
JournalJournal of virology
Volume74
Issue number20
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'DNA vaccination prevents and/or delays carcinoma development of papillomavirus-induced skin papillomas on rabbits'. Together they form a unique fingerprint.

Cite this