Does nitric oxide buffer arterial blood pressure variability in humans?

William H. Cooke, Rong Zhang, Julie H. Zuckerman, Jian Cui, Thad E. Wilson, Craig G. Crandall, Benjamin D. Levine

Research output: Contribution to journalArticle

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Abstract

Animal studies suggest that nitric oxide (NO) plays an important role in buffering short-term arterial pressure variability, but data from humans addressing this hypothesis are scarce. We evaluated the effects of NO synthase (NOS) inhibition on arterial blood pressure (BP) variability in eight healthy subjects in the supine position and during 60° head-up tilt (HUT). Systemic NOS was blocked by intravenous infusion of N G -monomethyl-L-arginine (L-NMMA). Electrocardiogram and beat-by-beat BP in the finger (Finapres) were recorded continuously for 6 min, and brachial cuff BP was recorded before and after L-NMMA in each body position. BP and R-R variability and their transfer functions were quantified by power spectral analysis in the low-frequency (LF; 0.05-0.15 Hz) and high-frequency (HF; 0.15-0.35 Hz) ranges. L-NMMA infusion increased supine BP (systolic, 109 ± 4 vs. 122 ± 3 mmHg, P = 0.03; diastolic, 68 ± 2 vs. 78 ± 3 mmHg, P = 0.002), but it did not affect supine R-R interval or BP variability. Before L-NMMA, HUT decreased HF R-R variability (P = 0.03), decreased transfer function gain (LF, 12 ± 2 vs. 5 ± 1 ms/mmHg, P = 0.007; HF, 18 ± 3 vs. 3 ± 1 ms/mmHg, P = 0.002), and increased LF BP variability (P < 0.0001). After L-NMMA, HUT resulted in similar changes in BP and R-R variability compared with tilt without L-NMMA. Increased supine BP after L-NMMA with no effect on BP variability during HUT suggests that tonic release of NO is important for systemic vascular tone and thus steady-state arterial pressure, but NO does not buffer dynamic BP oscillations in humans.

Original languageEnglish (US)
Pages (from-to)1466-1470
Number of pages5
JournalJournal of Applied Physiology
Volume93
Issue number4
DOIs
StatePublished - Jan 1 2002

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omega-N-Methylarginine
Arterial Pressure
Buffers
Nitric Oxide
Blood Pressure
Head
Nitric Oxide Synthase
Supine Position
Intravenous Infusions
Fingers
Blood Vessels
Arginine
Healthy Volunteers
Electrocardiography
Arm

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Cooke, W. H., Zhang, R., Zuckerman, J. H., Cui, J., Wilson, T. E., Crandall, C. G., & Levine, B. D. (2002). Does nitric oxide buffer arterial blood pressure variability in humans? Journal of Applied Physiology, 93(4), 1466-1470. https://doi.org/10.1152/japplphysiol.00287.2002
Cooke, William H. ; Zhang, Rong ; Zuckerman, Julie H. ; Cui, Jian ; Wilson, Thad E. ; Crandall, Craig G. ; Levine, Benjamin D. / Does nitric oxide buffer arterial blood pressure variability in humans?. In: Journal of Applied Physiology. 2002 ; Vol. 93, No. 4. pp. 1466-1470.
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Cooke, WH, Zhang, R, Zuckerman, JH, Cui, J, Wilson, TE, Crandall, CG & Levine, BD 2002, 'Does nitric oxide buffer arterial blood pressure variability in humans?', Journal of Applied Physiology, vol. 93, no. 4, pp. 1466-1470. https://doi.org/10.1152/japplphysiol.00287.2002

Does nitric oxide buffer arterial blood pressure variability in humans? / Cooke, William H.; Zhang, Rong; Zuckerman, Julie H.; Cui, Jian; Wilson, Thad E.; Crandall, Craig G.; Levine, Benjamin D.

In: Journal of Applied Physiology, Vol. 93, No. 4, 01.01.2002, p. 1466-1470.

Research output: Contribution to journalArticle

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T1 - Does nitric oxide buffer arterial blood pressure variability in humans?

AU - Cooke, William H.

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AU - Zuckerman, Julie H.

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