Double-stranded locked nucleic acid probes for revealing intracellular gene expression dynamics of cancer cells near mechanical wounds

Reza Riahi, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a double-stranded locked nucleic acid (dsLNA) probe for monitoring the intracellular gene expression toward the investigation of the role of mechanical injury in cancer metastasis. Specifically, the dsLNA probe is shown to allow real-time detection of intracellular mRNA in living cells. Using the dsLNA probes, the spatiotemporal gene expressions of cancer cells after mechanical injury are monitored for days. We show that the release of contact inhibition is sufficient to induce the migratory behavior of cancer cells while mechanical injury triggers the cellular antioxidant response in a region ~100 μm away from the wound.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages109-111
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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