Down-regulation of 14-3-3 isoforms and annexin A5 proteins in lung adenocarcinoma induced by the tobacco-specific nitrosamine NNK in the A/J mouse revealed by proteomic analysis

James D. Bortner, Arunangshu Das, Todd M. Umstead, Williard M. Freeman, Richard Somiari, Cesar Aliaga, David Phelps, Karam El-Bayoumy

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Abstract

The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone (NNK) is a potent lung carcinogen in the A/J mouse model. Here we identified and validated, using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry and immunoblotting, proteins that are differentially expressed in the lungs of mice treated with NNK versus vehicle control treatment. We also determined whether protein levels in the lungs of NNK-treated mice could be further modulated by the chemopreventive agent 1,4-phenylenebis(methylene)selenocyanate (p-XSC). The proteins identified in this study are SEC14-like 3, dihydropyrimidinase-like 2, proteasome subunit α type 5, annexin A5, 14-3-3 protein isoforms (θ, ε, σ, and ζ), Rho GDP dissociation inhibitor α, myosin light polypeptide 6, tubulin-α-1, vimentin, Atp5b protein, α-1-antitrypsin, and Clara cell 10 kDa protein (CC10). Among those proteins, we demonstrated for the first time that 14-3-3 isoforms (θ, ε, and σ) and annexin A5 were significantly down-regulated in mouse lung adenocarcinoma induced by NNK and were recovered by p-XSC. These proteins are involved in a variety of biological functions that are critical in lung carcinogenesis. Identification of these proteins in surrogate tissue in future studies would be highly useful in early detection of lung adenocarcinoma and clinical chemoprevention trials.

Original languageEnglish (US)
Pages (from-to)4050-4061
Number of pages12
JournalJournal of Proteome Research
Volume8
Issue number8
DOIs
StatePublished - Aug 7 2009

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Nitrosamines
Tobacco
Annexin A5
Proteomics
Protein Isoforms
Down-Regulation
Proteins
Two-Dimensional Difference Gel Electrophoresis
Lung
dihydropyrimidinase
Guanine Nucleotide Dissociation Inhibitors
14-3-3 Proteins
Adenocarcinoma of lung
Chemoprevention
Vimentin
Proteasome Endopeptidase Complex
Myosins
Tubulin
Electrophoresis
Immunoblotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

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title = "Down-regulation of 14-3-3 isoforms and annexin A5 proteins in lung adenocarcinoma induced by the tobacco-specific nitrosamine NNK in the A/J mouse revealed by proteomic analysis",
abstract = "The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone (NNK) is a potent lung carcinogen in the A/J mouse model. Here we identified and validated, using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry and immunoblotting, proteins that are differentially expressed in the lungs of mice treated with NNK versus vehicle control treatment. We also determined whether protein levels in the lungs of NNK-treated mice could be further modulated by the chemopreventive agent 1,4-phenylenebis(methylene)selenocyanate (p-XSC). The proteins identified in this study are SEC14-like 3, dihydropyrimidinase-like 2, proteasome subunit α type 5, annexin A5, 14-3-3 protein isoforms (θ, ε, σ, and ζ), Rho GDP dissociation inhibitor α, myosin light polypeptide 6, tubulin-α-1, vimentin, Atp5b protein, α-1-antitrypsin, and Clara cell 10 kDa protein (CC10). Among those proteins, we demonstrated for the first time that 14-3-3 isoforms (θ, ε, and σ) and annexin A5 were significantly down-regulated in mouse lung adenocarcinoma induced by NNK and were recovered by p-XSC. These proteins are involved in a variety of biological functions that are critical in lung carcinogenesis. Identification of these proteins in surrogate tissue in future studies would be highly useful in early detection of lung adenocarcinoma and clinical chemoprevention trials.",
author = "Bortner, {James D.} and Arunangshu Das and Umstead, {Todd M.} and Freeman, {Williard M.} and Richard Somiari and Cesar Aliaga and David Phelps and Karam El-Bayoumy",
year = "2009",
month = "8",
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language = "English (US)",
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T1 - Down-regulation of 14-3-3 isoforms and annexin A5 proteins in lung adenocarcinoma induced by the tobacco-specific nitrosamine NNK in the A/J mouse revealed by proteomic analysis

AU - Bortner, James D.

AU - Das, Arunangshu

AU - Umstead, Todd M.

AU - Freeman, Williard M.

AU - Somiari, Richard

AU - Aliaga, Cesar

AU - Phelps, David

AU - El-Bayoumy, Karam

PY - 2009/8/7

Y1 - 2009/8/7

N2 - The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone (NNK) is a potent lung carcinogen in the A/J mouse model. Here we identified and validated, using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry and immunoblotting, proteins that are differentially expressed in the lungs of mice treated with NNK versus vehicle control treatment. We also determined whether protein levels in the lungs of NNK-treated mice could be further modulated by the chemopreventive agent 1,4-phenylenebis(methylene)selenocyanate (p-XSC). The proteins identified in this study are SEC14-like 3, dihydropyrimidinase-like 2, proteasome subunit α type 5, annexin A5, 14-3-3 protein isoforms (θ, ε, σ, and ζ), Rho GDP dissociation inhibitor α, myosin light polypeptide 6, tubulin-α-1, vimentin, Atp5b protein, α-1-antitrypsin, and Clara cell 10 kDa protein (CC10). Among those proteins, we demonstrated for the first time that 14-3-3 isoforms (θ, ε, and σ) and annexin A5 were significantly down-regulated in mouse lung adenocarcinoma induced by NNK and were recovered by p-XSC. These proteins are involved in a variety of biological functions that are critical in lung carcinogenesis. Identification of these proteins in surrogate tissue in future studies would be highly useful in early detection of lung adenocarcinoma and clinical chemoprevention trials.

AB - The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone (NNK) is a potent lung carcinogen in the A/J mouse model. Here we identified and validated, using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry and immunoblotting, proteins that are differentially expressed in the lungs of mice treated with NNK versus vehicle control treatment. We also determined whether protein levels in the lungs of NNK-treated mice could be further modulated by the chemopreventive agent 1,4-phenylenebis(methylene)selenocyanate (p-XSC). The proteins identified in this study are SEC14-like 3, dihydropyrimidinase-like 2, proteasome subunit α type 5, annexin A5, 14-3-3 protein isoforms (θ, ε, σ, and ζ), Rho GDP dissociation inhibitor α, myosin light polypeptide 6, tubulin-α-1, vimentin, Atp5b protein, α-1-antitrypsin, and Clara cell 10 kDa protein (CC10). Among those proteins, we demonstrated for the first time that 14-3-3 isoforms (θ, ε, and σ) and annexin A5 were significantly down-regulated in mouse lung adenocarcinoma induced by NNK and were recovered by p-XSC. These proteins are involved in a variety of biological functions that are critical in lung carcinogenesis. Identification of these proteins in surrogate tissue in future studies would be highly useful in early detection of lung adenocarcinoma and clinical chemoprevention trials.

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