Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

Franklin D. Fuller, Sheraz Gul, Ruchira Chatterjee, E. Sethe Burgie, Iris D. Young, Hugo Lebrette, Vivek Srinivas, Aaron S. Brewster, Tara Michels-Clark, Jonathan A. Clinger, Babak Andi, Mohamed Ibrahim, Ernest Pastor, Casper De Lichtenberg, Rana Hussein, Christopher J. Pollock, Miao Zhang, Claudiu A. Stan, Thomas Kroll, Thomas FranssonClemens Weninger, Markus Kubin, Pierre Aller, Louise Lassalle, Philipp Bräuer, Mitchell D. Miller, Muhamed Amin, Sergey Koroidov, Christian G. Roessler, Marc Allaire, Raymond G. Sierra, Peter T. Docker, James M. Glownia, Silke Nelson, Jason E. Koglin, Diling Zhu, Matthieu Chollet, Sanghoon Song, Henrik Lemke, Mengning Liang, Dimosthenis Sokaras, Roberto Alonso-Mori, Athina Zouni, Johannes Messinger, Uwe Bergmann, Amie Kathleen Boal, Joseph M. Bollinger, Jr., Carsten Krebs, Martin Högbom, George N. Phillips, Richard D. Vierstra, Nicholas K. Sauter, Allen M. Orville, Jan Kern, Vittal K. Yachandra, Junko Yano

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. the implementation of such a multimodal approach can be compromised by con?icting requirements to optimize each individual method. in particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. studies with photosystem ii, the phytochrome photoreceptor, and ribonucleotide reductase r2 illustrate the power and versatility of this method.

Original languageEnglish (US)
Pages (from-to)443-449
Number of pages7
JournalNature methods
Volume14
Issue number4
DOIs
StatePublished - Feb 27 2017

Fingerprint

Belt drives
Phytochrome
X ray lasers
Ribonucleotide Reductases
Crystallography
Photochemical reactions
X ray crystallography
Free electron lasers
Macromolecules
Chemical properties
Light sources
Chemical reactions
Lasers
Acoustics
X-Rays
Spectroscopy
Electrons
Enzymes
Proteins
X-Ray Emission Spectrometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fuller, F. D., Gul, S., Chatterjee, R., Sethe Burgie, E., Young, I. D., Lebrette, H., ... Yano, J. (2017). Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nature methods, 14(4), 443-449. https://doi.org/10.1038/nmeth.4195
Fuller, Franklin D. ; Gul, Sheraz ; Chatterjee, Ruchira ; Sethe Burgie, E. ; Young, Iris D. ; Lebrette, Hugo ; Srinivas, Vivek ; Brewster, Aaron S. ; Michels-Clark, Tara ; Clinger, Jonathan A. ; Andi, Babak ; Ibrahim, Mohamed ; Pastor, Ernest ; De Lichtenberg, Casper ; Hussein, Rana ; Pollock, Christopher J. ; Zhang, Miao ; Stan, Claudiu A. ; Kroll, Thomas ; Fransson, Thomas ; Weninger, Clemens ; Kubin, Markus ; Aller, Pierre ; Lassalle, Louise ; Bräuer, Philipp ; Miller, Mitchell D. ; Amin, Muhamed ; Koroidov, Sergey ; Roessler, Christian G. ; Allaire, Marc ; Sierra, Raymond G. ; Docker, Peter T. ; Glownia, James M. ; Nelson, Silke ; Koglin, Jason E. ; Zhu, Diling ; Chollet, Matthieu ; Song, Sanghoon ; Lemke, Henrik ; Liang, Mengning ; Sokaras, Dimosthenis ; Alonso-Mori, Roberto ; Zouni, Athina ; Messinger, Johannes ; Bergmann, Uwe ; Boal, Amie Kathleen ; Bollinger, Jr., Joseph M. ; Krebs, Carsten ; Högbom, Martin ; Phillips, George N. ; Vierstra, Richard D. ; Sauter, Nicholas K. ; Orville, Allen M. ; Kern, Jan ; Yachandra, Vittal K. ; Yano, Junko. / Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. In: Nature methods. 2017 ; Vol. 14, No. 4. pp. 443-449.
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Fuller, FD, Gul, S, Chatterjee, R, Sethe Burgie, E, Young, ID, Lebrette, H, Srinivas, V, Brewster, AS, Michels-Clark, T, Clinger, JA, Andi, B, Ibrahim, M, Pastor, E, De Lichtenberg, C, Hussein, R, Pollock, CJ, Zhang, M, Stan, CA, Kroll, T, Fransson, T, Weninger, C, Kubin, M, Aller, P, Lassalle, L, Bräuer, P, Miller, MD, Amin, M, Koroidov, S, Roessler, CG, Allaire, M, Sierra, RG, Docker, PT, Glownia, JM, Nelson, S, Koglin, JE, Zhu, D, Chollet, M, Song, S, Lemke, H, Liang, M, Sokaras, D, Alonso-Mori, R, Zouni, A, Messinger, J, Bergmann, U, Boal, AK, Bollinger, Jr., JM, Krebs, C, Högbom, M, Phillips, GN, Vierstra, RD, Sauter, NK, Orville, AM, Kern, J, Yachandra, VK & Yano, J 2017, 'Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers', Nature methods, vol. 14, no. 4, pp. 443-449. https://doi.org/10.1038/nmeth.4195

Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. / Fuller, Franklin D.; Gul, Sheraz; Chatterjee, Ruchira; Sethe Burgie, E.; Young, Iris D.; Lebrette, Hugo; Srinivas, Vivek; Brewster, Aaron S.; Michels-Clark, Tara; Clinger, Jonathan A.; Andi, Babak; Ibrahim, Mohamed; Pastor, Ernest; De Lichtenberg, Casper; Hussein, Rana; Pollock, Christopher J.; Zhang, Miao; Stan, Claudiu A.; Kroll, Thomas; Fransson, Thomas; Weninger, Clemens; Kubin, Markus; Aller, Pierre; Lassalle, Louise; Bräuer, Philipp; Miller, Mitchell D.; Amin, Muhamed; Koroidov, Sergey; Roessler, Christian G.; Allaire, Marc; Sierra, Raymond G.; Docker, Peter T.; Glownia, James M.; Nelson, Silke; Koglin, Jason E.; Zhu, Diling; Chollet, Matthieu; Song, Sanghoon; Lemke, Henrik; Liang, Mengning; Sokaras, Dimosthenis; Alonso-Mori, Roberto; Zouni, Athina; Messinger, Johannes; Bergmann, Uwe; Boal, Amie Kathleen; Bollinger, Jr., Joseph M.; Krebs, Carsten; Högbom, Martin; Phillips, George N.; Vierstra, Richard D.; Sauter, Nicholas K.; Orville, Allen M.; Kern, Jan; Yachandra, Vittal K.; Yano, Junko.

In: Nature methods, Vol. 14, No. 4, 27.02.2017, p. 443-449.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

AU - Fuller, Franklin D.

AU - Gul, Sheraz

AU - Chatterjee, Ruchira

AU - Sethe Burgie, E.

AU - Young, Iris D.

AU - Lebrette, Hugo

AU - Srinivas, Vivek

AU - Brewster, Aaron S.

AU - Michels-Clark, Tara

AU - Clinger, Jonathan A.

AU - Andi, Babak

AU - Ibrahim, Mohamed

AU - Pastor, Ernest

AU - De Lichtenberg, Casper

AU - Hussein, Rana

AU - Pollock, Christopher J.

AU - Zhang, Miao

AU - Stan, Claudiu A.

AU - Kroll, Thomas

AU - Fransson, Thomas

AU - Weninger, Clemens

AU - Kubin, Markus

AU - Aller, Pierre

AU - Lassalle, Louise

AU - Bräuer, Philipp

AU - Miller, Mitchell D.

AU - Amin, Muhamed

AU - Koroidov, Sergey

AU - Roessler, Christian G.

AU - Allaire, Marc

AU - Sierra, Raymond G.

AU - Docker, Peter T.

AU - Glownia, James M.

AU - Nelson, Silke

AU - Koglin, Jason E.

AU - Zhu, Diling

AU - Chollet, Matthieu

AU - Song, Sanghoon

AU - Lemke, Henrik

AU - Liang, Mengning

AU - Sokaras, Dimosthenis

AU - Alonso-Mori, Roberto

AU - Zouni, Athina

AU - Messinger, Johannes

AU - Bergmann, Uwe

AU - Boal, Amie Kathleen

AU - Bollinger, Jr., Joseph M.

AU - Krebs, Carsten

AU - Högbom, Martin

AU - Phillips, George N.

AU - Vierstra, Richard D.

AU - Sauter, Nicholas K.

AU - Orville, Allen M.

AU - Kern, Jan

AU - Yachandra, Vittal K.

AU - Yano, Junko

PY - 2017/2/27

Y1 - 2017/2/27

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AB - X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. the implementation of such a multimodal approach can be compromised by con?icting requirements to optimize each individual method. in particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. studies with photosystem ii, the phytochrome photoreceptor, and ribonucleotide reductase r2 illustrate the power and versatility of this method.

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Fuller FD, Gul S, Chatterjee R, Sethe Burgie E, Young ID, Lebrette H et al. Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nature methods. 2017 Feb 27;14(4):443-449. https://doi.org/10.1038/nmeth.4195