Drosophila p120 catenin plays a supporting role in cell adhesion but is not an essential adherens junction component

Steven H. Myster, Robert Cavallo, Charles T. Anderson, Donald T. Fox, Mark Peifer

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Cadherin-catenin complexes, localized to adherens junctions, are essential for cell-cell adhesion. One means of regulating adhesion is through the juxtamembrane domain of the cadherin cytoplasmic tail. This region is the binding site for p120, leading to the hypothesis that p120 is a key regulator of cell adhesion. p120 has also been suggested to regulate the GTPase Rho and to regulate transcription via its binding partner Kaiso. To test these hypothesized functions, we turned to Drosophila, which has only a single p120 family member. It localizes to adherens junctions and binds the juxtamembrane region of DE-cadherin (DE-cad). We generated null alleles of p120 and found that mutants are viable and fertile and have no substantial changes in junction structure or function. However, p120 mutations strongly enhance mutations in the genes encoding DE-cadherin or Armadillo, the β-catenin homologue. Finally, we examined the localization of p120 during embryogenesis. p120 localizes to adherens junctions, but its localization there is less universal than that of core adherens junction proteins. Together, these data suggest that p120 is an important positive modulator of adhesion but that it is not an essential core component of adherens junctions.

Original languageEnglish (US)
Pages (from-to)433-449
Number of pages17
JournalJournal of Cell Biology
Volume160
Issue number3
DOIs
StatePublished - Feb 3 2003

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Adherens Junctions
Cell Adhesion
Drosophila
Cadherins
Catenins
Armadillos
rho GTP-Binding Proteins
Mutation
Embryonic Development
Tail
Alleles
Binding Sites
delta catenin
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Myster, Steven H. ; Cavallo, Robert ; Anderson, Charles T. ; Fox, Donald T. ; Peifer, Mark. / Drosophila p120 catenin plays a supporting role in cell adhesion but is not an essential adherens junction component. In: Journal of Cell Biology. 2003 ; Vol. 160, No. 3. pp. 433-449.
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Drosophila p120 catenin plays a supporting role in cell adhesion but is not an essential adherens junction component. / Myster, Steven H.; Cavallo, Robert; Anderson, Charles T.; Fox, Donald T.; Peifer, Mark.

In: Journal of Cell Biology, Vol. 160, No. 3, 03.02.2003, p. 433-449.

Research output: Contribution to journalArticle

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