Dual growth factor releasing multi-functional nanofibers for wound healing

Zhiwei Xie, Christian B. Paras, Hong Weng, Primana Punnakitikashem, Lee Chun Su, Khanh Vu, Liping Tang, Jian Yang, Kytai T. Nguyen

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The objective of this research is to develop a dual growth factor-releasing nanoparticle-in-nanofiber system for wound healing applications. In order to mimic and promote the natural healing procedure, chitosan and poly(ethylene oxide) were electrospun into nanofibrous meshes as mimics of extracellular matrix. Vascular endothelial growth factor (VEGF) was loaded within nanofibers to promote angiogenesis in the short term. In addition, platelet-derived growth factor-BB (PDGF-BB) encapsulated poly(lactic-co-glycolic acid) nanoparticles were embedded inside nanofibers to generate a sustained release of PDGF-BB for accelerated tissue regeneration and remodeling. In vitro studies revealed that our nanofibrous composites delivered VEGF quickly and PDGF-BB in a relayed manner, supported fibroblast growth and exhibited anti-bacterial activities. A preliminary in vivo study performed on normal full thickness rat skin wound models demonstrated that nanofiber/nanoparticle scaffolds significantly accelerated the wound healing process by promoting angiogenesis, increasing re-epithelialization and controlling granulation tissue formation. For later stages of healing, evidence also showed quicker collagen deposition and earlier remodeling of the injured site to achieve a faster full regeneration of skin compared to the commercial Hydrofera Blue® wound dressing. These results suggest that our nanoparticle-in-nanofiber system could provide a promising treatment for normal and chronic wound healing.

Original languageEnglish (US)
Pages (from-to)9351-9359
Number of pages9
JournalActa Biomaterialia
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2013

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Nanofibers
Wound Healing
Intercellular Signaling Peptides and Proteins
Nanoparticles
Platelets
Vascular Endothelial Growth Factor A
Regeneration
Skin
Re-Epithelialization
Ethylene Oxide
Tissue regeneration
Granulation
Granulation Tissue
Wounds and Injuries
Chitosan
Fibroblasts
Scaffolds (biology)
Bandages
Polyethylene oxides
Extracellular Matrix

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Xie, Z., Paras, C. B., Weng, H., Punnakitikashem, P., Su, L. C., Vu, K., ... Nguyen, K. T. (2013). Dual growth factor releasing multi-functional nanofibers for wound healing. Acta Biomaterialia, 9(12), 9351-9359. https://doi.org/10.1016/j.actbio.2013.07.030
Xie, Zhiwei ; Paras, Christian B. ; Weng, Hong ; Punnakitikashem, Primana ; Su, Lee Chun ; Vu, Khanh ; Tang, Liping ; Yang, Jian ; Nguyen, Kytai T. / Dual growth factor releasing multi-functional nanofibers for wound healing. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 12. pp. 9351-9359.
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Xie, Z, Paras, CB, Weng, H, Punnakitikashem, P, Su, LC, Vu, K, Tang, L, Yang, J & Nguyen, KT 2013, 'Dual growth factor releasing multi-functional nanofibers for wound healing', Acta Biomaterialia, vol. 9, no. 12, pp. 9351-9359. https://doi.org/10.1016/j.actbio.2013.07.030

Dual growth factor releasing multi-functional nanofibers for wound healing. / Xie, Zhiwei; Paras, Christian B.; Weng, Hong; Punnakitikashem, Primana; Su, Lee Chun; Vu, Khanh; Tang, Liping; Yang, Jian; Nguyen, Kytai T.

In: Acta Biomaterialia, Vol. 9, No. 12, 01.12.2013, p. 9351-9359.

Research output: Contribution to journalArticle

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AU - Xie, Zhiwei

AU - Paras, Christian B.

AU - Weng, Hong

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AU - Vu, Khanh

AU - Tang, Liping

AU - Yang, Jian

AU - Nguyen, Kytai T.

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Xie Z, Paras CB, Weng H, Punnakitikashem P, Su LC, Vu K et al. Dual growth factor releasing multi-functional nanofibers for wound healing. Acta Biomaterialia. 2013 Dec 1;9(12):9351-9359. https://doi.org/10.1016/j.actbio.2013.07.030