Abstract

Plasma samples from pediatric cardiac patients undergoing cardiopulmonary bypass (CPB) procedures were used to identify and characterize patterns of changes in potential biomarkers related to tissue damage and inflammation. These included proteins associated with systemic inflammatory response syndrome. Potential biomarkers were identified using a dual-platform proteomics approach requiring ∼150 μL of plasma, which included two-dimensional difference gel electrophoresis (2D-DIGE) and a multiplexed immunoassay. Methods used in the dual approach measured levels of 129 proteins in plasma from pediatric CPB patients. Of these, 70 proteins changed significantly (p < 0.05) between time points, and 36 of these retained significance after the highly stringent Bonferroni correction [p < 0.001 for 2D-DIGE and p < 0.00056 for multianalyte profile (MAP) assays]. Many of the changing proteins were associated with tissue damage, inflammation, and oxidative stress. This study uses a novel approach that combines two discovery proteomics techniques to identify a pattern of potential biomarkers changing after CPB. This approach required only 150 μL of plasma per time point and provided quantitative information on 129 proteins. The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients.

Original languageEnglish (US)
Pages (from-to)641-649
Number of pages9
JournalPediatric Research
Volume67
Issue number6
DOIs
StatePublished - Jun 1 2010

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Cardiopulmonary Bypass
Proteomics
Two-Dimensional Difference Gel Electrophoresis
Biomarkers
Pediatrics
Inflammation
Proteins
Systemic Inflammatory Response Syndrome
Immunoassay
Blood Proteins
Oxidative Stress
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

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title = "Dual-platform proteomics study of plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass",
abstract = "Plasma samples from pediatric cardiac patients undergoing cardiopulmonary bypass (CPB) procedures were used to identify and characterize patterns of changes in potential biomarkers related to tissue damage and inflammation. These included proteins associated with systemic inflammatory response syndrome. Potential biomarkers were identified using a dual-platform proteomics approach requiring ∼150 μL of plasma, which included two-dimensional difference gel electrophoresis (2D-DIGE) and a multiplexed immunoassay. Methods used in the dual approach measured levels of 129 proteins in plasma from pediatric CPB patients. Of these, 70 proteins changed significantly (p < 0.05) between time points, and 36 of these retained significance after the highly stringent Bonferroni correction [p < 0.001 for 2D-DIGE and p < 0.00056 for multianalyte profile (MAP) assays]. Many of the changing proteins were associated with tissue damage, inflammation, and oxidative stress. This study uses a novel approach that combines two discovery proteomics techniques to identify a pattern of potential biomarkers changing after CPB. This approach required only 150 μL of plasma per time point and provided quantitative information on 129 proteins. The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients.",
author = "Umstead, {Todd M.} and Lu, {Chia Jung K.} and Freeman, {Willard M.} and Myers, {John L.} and Clark, {J. Brian} and Thomas, {Neal J.} and Chinchilli, {Vernon M.} and Vrana, {Kent E.} and Akif {\"U}ndar and Phelps, {David S.}",
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T1 - Dual-platform proteomics study of plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass

AU - Umstead, Todd M.

AU - Lu, Chia Jung K.

AU - Freeman, Willard M.

AU - Myers, John L.

AU - Clark, J. Brian

AU - Thomas, Neal J.

AU - Chinchilli, Vernon M.

AU - Vrana, Kent E.

AU - Ündar, Akif

AU - Phelps, David S.

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Plasma samples from pediatric cardiac patients undergoing cardiopulmonary bypass (CPB) procedures were used to identify and characterize patterns of changes in potential biomarkers related to tissue damage and inflammation. These included proteins associated with systemic inflammatory response syndrome. Potential biomarkers were identified using a dual-platform proteomics approach requiring ∼150 μL of plasma, which included two-dimensional difference gel electrophoresis (2D-DIGE) and a multiplexed immunoassay. Methods used in the dual approach measured levels of 129 proteins in plasma from pediatric CPB patients. Of these, 70 proteins changed significantly (p < 0.05) between time points, and 36 of these retained significance after the highly stringent Bonferroni correction [p < 0.001 for 2D-DIGE and p < 0.00056 for multianalyte profile (MAP) assays]. Many of the changing proteins were associated with tissue damage, inflammation, and oxidative stress. This study uses a novel approach that combines two discovery proteomics techniques to identify a pattern of potential biomarkers changing after CPB. This approach required only 150 μL of plasma per time point and provided quantitative information on 129 proteins. The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients.

AB - Plasma samples from pediatric cardiac patients undergoing cardiopulmonary bypass (CPB) procedures were used to identify and characterize patterns of changes in potential biomarkers related to tissue damage and inflammation. These included proteins associated with systemic inflammatory response syndrome. Potential biomarkers were identified using a dual-platform proteomics approach requiring ∼150 μL of plasma, which included two-dimensional difference gel electrophoresis (2D-DIGE) and a multiplexed immunoassay. Methods used in the dual approach measured levels of 129 proteins in plasma from pediatric CPB patients. Of these, 70 proteins changed significantly (p < 0.05) between time points, and 36 of these retained significance after the highly stringent Bonferroni correction [p < 0.001 for 2D-DIGE and p < 0.00056 for multianalyte profile (MAP) assays]. Many of the changing proteins were associated with tissue damage, inflammation, and oxidative stress. This study uses a novel approach that combines two discovery proteomics techniques to identify a pattern of potential biomarkers changing after CPB. This approach required only 150 μL of plasma per time point and provided quantitative information on 129 proteins. The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients.

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