Dynamic analysis of reactive oxygen nitrogen species in plasma-activated culture medium by UV absorption spectroscopy

Timothy R. Brubaker, Kenji Ishikawa, Keigo Takeda, Jun Seok Oh, Hiroki Kondo, Hiroshi Hashizume, Hiromasa Tanaka, Sean D. Knecht, Sven G. Bilén, Masaru Hori

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Abstract

The liquid-phase chemical kinetics of a cell culture basal medium during treatment by an argon-fed, non-equilibrium atmospheric-pressure plasma source were investigated using real-time ultraviolet absorption spectroscopy and colorimetric assays. Depth- and time-resolved NO2- and NO3- concentrations were strongly inhomogeneous and primarily driven by convection during and after plasma-liquid interactions. H2O2 concentrations determined from deconvolved optical depth spectra were found to compensate for the optical depth spectra of excluded reactive species and changes in dissolved gas content. Plasma-activated media remained weakly basic due to NaHCO3 buffering, preventing the H+-catalyzed decomposition of NO2- seen in acidic plasma-activated water. An initial increase in pH may indicate CO2 sparging. Furthermore, the pH-dependency of UV optical depth spectra illustrated the need for pH compensation in the fitting of optical depth data.

Original languageEnglish (US)
Article number213301
JournalJournal of Applied Physics
Volume122
Issue number21
DOIs
StatePublished - Dec 7 2017

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Brubaker, T. R., Ishikawa, K., Takeda, K., Oh, J. S., Kondo, H., Hashizume, H., Tanaka, H., Knecht, S. D., Bilén, S. G., & Hori, M. (2017). Dynamic analysis of reactive oxygen nitrogen species in plasma-activated culture medium by UV absorption spectroscopy. Journal of Applied Physics, 122(21), [213301]. https://doi.org/10.1063/1.4999256