Dynamic changes in pod and fungal physiology associated with the shift from biotrophy to necrotrophy during the infection of Theobroma cacao by Moniliophthora roreri

Bryan A. Bailey, Jayne Crozier, Richard C. Sicher, Mary D. Strem, Rachel Melnick, Marcelo F. Carazzolle, Gustavo G.L. Costa, Gonçalo A.G. Pereira, Dapeng Zhang, Siela Maximova, Mark Guiltinan, Lyndel Meinhardt

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Theobroma cacao pods were inoculated with meiospores of Moniliophthora roreri (Mr), a hemibiotrophic basidiomycete causing frosty pod rot. Pods were malformed 30 days after inoculation (DAI) and sporulation was observed 60 DAI. Glucose and asparagine concentrations decreased and mannitol and malonate increased in infected pods 30 DAI. By 60 DAI, most carbohydrates, amino acids, and organic acids were drastically reduced by infection. Mannitol and succinic acid levels increased 60 DAI and likely originated from Mr. RT-qPCR analysis of cacao ESTs indicated a strong response to infection 30 DAI in malformed pod. Evidence indicated that biotrophic hyphae colonized pods and a shift to necrotrophic growth occurred later (during the end stages of infection). Expression of cacao ESTs associated with plant hormone biosynthesis and action was altered. Changes in the expression of Mr ESTs in response to nutrient deficiency in pure culture were small. Changes in Mr gene expression patterns and levels of specific metabolites in necrotic sporulating pods 60 DAI compared to malformed pods 30 DAI indicated that the glyoxylate cycle of Mr was up regulated during the shift from biotrophic to necrotrophic phases of the disease cycle.

Original languageEnglish (US)
Pages (from-to)84-96
Number of pages13
JournalPhysiological and Molecular Plant Pathology
Volume81
DOIs
StatePublished - Jan 1 2013

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fungus physiology
Moniliophthora roreri
Theobroma cacao
Cacao
Expressed Sequence Tags
pods
Mannitol
Infection
infection
Basidiomycota
Plant Growth Regulators
Hyphae
Asparagine
Succinic Acid
mannitol
Carbohydrates
Gene Expression
Amino Acids
Glucose
Food

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Bailey, Bryan A. ; Crozier, Jayne ; Sicher, Richard C. ; Strem, Mary D. ; Melnick, Rachel ; Carazzolle, Marcelo F. ; Costa, Gustavo G.L. ; Pereira, Gonçalo A.G. ; Zhang, Dapeng ; Maximova, Siela ; Guiltinan, Mark ; Meinhardt, Lyndel. / Dynamic changes in pod and fungal physiology associated with the shift from biotrophy to necrotrophy during the infection of Theobroma cacao by Moniliophthora roreri. In: Physiological and Molecular Plant Pathology. 2013 ; Vol. 81. pp. 84-96.
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abstract = "Theobroma cacao pods were inoculated with meiospores of Moniliophthora roreri (Mr), a hemibiotrophic basidiomycete causing frosty pod rot. Pods were malformed 30 days after inoculation (DAI) and sporulation was observed 60 DAI. Glucose and asparagine concentrations decreased and mannitol and malonate increased in infected pods 30 DAI. By 60 DAI, most carbohydrates, amino acids, and organic acids were drastically reduced by infection. Mannitol and succinic acid levels increased 60 DAI and likely originated from Mr. RT-qPCR analysis of cacao ESTs indicated a strong response to infection 30 DAI in malformed pod. Evidence indicated that biotrophic hyphae colonized pods and a shift to necrotrophic growth occurred later (during the end stages of infection). Expression of cacao ESTs associated with plant hormone biosynthesis and action was altered. Changes in the expression of Mr ESTs in response to nutrient deficiency in pure culture were small. Changes in Mr gene expression patterns and levels of specific metabolites in necrotic sporulating pods 60 DAI compared to malformed pods 30 DAI indicated that the glyoxylate cycle of Mr was up regulated during the shift from biotrophic to necrotrophic phases of the disease cycle.",
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Dynamic changes in pod and fungal physiology associated with the shift from biotrophy to necrotrophy during the infection of Theobroma cacao by Moniliophthora roreri. / Bailey, Bryan A.; Crozier, Jayne; Sicher, Richard C.; Strem, Mary D.; Melnick, Rachel; Carazzolle, Marcelo F.; Costa, Gustavo G.L.; Pereira, Gonçalo A.G.; Zhang, Dapeng; Maximova, Siela; Guiltinan, Mark; Meinhardt, Lyndel.

In: Physiological and Molecular Plant Pathology, Vol. 81, 01.01.2013, p. 84-96.

Research output: Contribution to journalArticle

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AU - Bailey, Bryan A.

AU - Crozier, Jayne

AU - Sicher, Richard C.

AU - Strem, Mary D.

AU - Melnick, Rachel

AU - Carazzolle, Marcelo F.

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AU - Zhang, Dapeng

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AU - Guiltinan, Mark

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