Dynamics of hydrated polyurethane biomaterials

Surface microphase restructuring, protein activity and platelet adhesion

Lichong Xu, James Patrick Runt, Christopher Siedlecki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Microphase separation is a central feature of segmented polyurethane biomaterials and contributes to the biological response to these materials. In this study we utilized atomic force microscopy (AFM) to study the dynamic restructuring of three polyurethanes having soft segment chemistries of interest in biomedical applications. For each of the materials we followed the changes in near surface mechanical properties during hydration, as well as fibrinogen activity and platelet adhesion on these surfaces. Both AFM phase imaging and force mode analysis demonstrated that these polyurethane biomaterials underwent reorientation and rearrangement resulting in a net enrichment of hard domains at the surface. Fibrinogen activity and platelet adhesion on the polyurethane surfaces were found to decrease with increasing hydration time. The findings suggest that water-induced enrichment of hydrophilic hard domains at the surface changes the local surface physical and chemical properties in a way that influences the conformation of fibrinogen, changing the availability of the platelet-binding sites in the protein. This work demonstrates that the hydrated polyurethane biomaterial interface is a complex and dynamic environment where the surface chemistry is changing, altering the activity of fibrinogen and affecting blood platelet adhesion.

Original languageEnglish (US)
Pages (from-to)1938-1947
Number of pages10
JournalActa Biomaterialia
Volume6
Issue number6
DOIs
StatePublished - Jan 1 2010

Fingerprint

Polyurethanes
Biocompatible Materials
Platelets
Biomaterials
Blood Platelets
Adhesion
Fibrinogen
Proteins
Atomic Force Microscopy
Hydration
Atomic force microscopy
Surface Properties
Microphase separation
Binding sites
Surface chemistry
Chemical properties
Binding Sites
Conformations
Blood
Physical properties

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

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abstract = "Microphase separation is a central feature of segmented polyurethane biomaterials and contributes to the biological response to these materials. In this study we utilized atomic force microscopy (AFM) to study the dynamic restructuring of three polyurethanes having soft segment chemistries of interest in biomedical applications. For each of the materials we followed the changes in near surface mechanical properties during hydration, as well as fibrinogen activity and platelet adhesion on these surfaces. Both AFM phase imaging and force mode analysis demonstrated that these polyurethane biomaterials underwent reorientation and rearrangement resulting in a net enrichment of hard domains at the surface. Fibrinogen activity and platelet adhesion on the polyurethane surfaces were found to decrease with increasing hydration time. The findings suggest that water-induced enrichment of hydrophilic hard domains at the surface changes the local surface physical and chemical properties in a way that influences the conformation of fibrinogen, changing the availability of the platelet-binding sites in the protein. This work demonstrates that the hydrated polyurethane biomaterial interface is a complex and dynamic environment where the surface chemistry is changing, altering the activity of fibrinogen and affecting blood platelet adhesion.",
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Dynamics of hydrated polyurethane biomaterials : Surface microphase restructuring, protein activity and platelet adhesion. / Xu, Lichong; Runt, James Patrick; Siedlecki, Christopher.

In: Acta Biomaterialia, Vol. 6, No. 6, 01.01.2010, p. 1938-1947.

Research output: Contribution to journalArticle

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