Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms

Anne Teissier, Corentin Le Magueresse, Jimmy Olusakin, Belmira L.S. Andrade da Costa, Angela M. De Stasi, Alberto Bacci, Yuka Imamura Kawasawa, Vidita A. Vaidya, Patricia Gaspar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Exposure to stress during early life (infancy/childhood) has long-term effects on the structure and function of the prefrontal cortex (PFC), and increases the risk for adult depression and anxiety disorders. However, little is known about the molecular and cellular mechanisms of these effects. Here, we focused on changes induced by chronic maternal separation during the first 2 weeks of postnatal life. Unbiased mRNA expression profiling in the medial PFC (mPFC) of maternally separated (MS) pups identified an increased expression of myelin-related genes and a decreased expression of immediate early genes. Oligodendrocyte lineage markers and birthdating experiments indicated a precocious oligodendrocyte differentiation in the mPFC at P15, leading to a depletion of the oligodendrocyte progenitor pool in MS adults. We tested the role of neuronal activity in oligodendrogenesis, using designed receptors exclusively activated by designed drugs (DREADDs) techniques. hM4Di or hM3Dq constructs were transfected into mPFC neurons using fast-acting AAV8 viruses. Reduction of mPFC neuron excitability during the first 2 postnatal weeks caused a premature differentiation of oligodendrocytes similar to the MS pups, while chemogenetic activation normalised it in the MS animals. Bidirectional manipulation of neuron excitability in the mPFC during the P2–P14 period had long lasting effects on adult emotional behaviours and on temporal object recognition: hM4Di mimicked MS effects, while hM3Dq prevented the pro-depressive effects and short-term memory impairment of MS. Thus, our results identify neuronal activity as a critical target of early-life stress and demonstrate its function in controlling both postnatal oligodendrogenesis and adult mPFC-related behaviours.

Original languageEnglish (US)
JournalMolecular Psychiatry
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Oligodendroglia
Psychological Stress
Prefrontal Cortex
Neurons
Immediate-Early Genes
Myelin Sheath
Anxiety Disorders
Short-Term Memory
Mothers
Depression
Viruses
Messenger RNA
Pharmaceutical Preparations
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Teissier, A., Le Magueresse, C., Olusakin, J., Andrade da Costa, B. L. S., De Stasi, A. M., Bacci, A., ... Gaspar, P. (Accepted/In press). Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms. Molecular Psychiatry. https://doi.org/10.1038/s41380-019-0493-2
Teissier, Anne ; Le Magueresse, Corentin ; Olusakin, Jimmy ; Andrade da Costa, Belmira L.S. ; De Stasi, Angela M. ; Bacci, Alberto ; Imamura Kawasawa, Yuka ; Vaidya, Vidita A. ; Gaspar, Patricia. / Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms. In: Molecular Psychiatry. 2019.
@article{756e98811f8941f881d500a02d26ff7b,
title = "Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms",
abstract = "Exposure to stress during early life (infancy/childhood) has long-term effects on the structure and function of the prefrontal cortex (PFC), and increases the risk for adult depression and anxiety disorders. However, little is known about the molecular and cellular mechanisms of these effects. Here, we focused on changes induced by chronic maternal separation during the first 2 weeks of postnatal life. Unbiased mRNA expression profiling in the medial PFC (mPFC) of maternally separated (MS) pups identified an increased expression of myelin-related genes and a decreased expression of immediate early genes. Oligodendrocyte lineage markers and birthdating experiments indicated a precocious oligodendrocyte differentiation in the mPFC at P15, leading to a depletion of the oligodendrocyte progenitor pool in MS adults. We tested the role of neuronal activity in oligodendrogenesis, using designed receptors exclusively activated by designed drugs (DREADDs) techniques. hM4Di or hM3Dq constructs were transfected into mPFC neurons using fast-acting AAV8 viruses. Reduction of mPFC neuron excitability during the first 2 postnatal weeks caused a premature differentiation of oligodendrocytes similar to the MS pups, while chemogenetic activation normalised it in the MS animals. Bidirectional manipulation of neuron excitability in the mPFC during the P2–P14 period had long lasting effects on adult emotional behaviours and on temporal object recognition: hM4Di mimicked MS effects, while hM3Dq prevented the pro-depressive effects and short-term memory impairment of MS. Thus, our results identify neuronal activity as a critical target of early-life stress and demonstrate its function in controlling both postnatal oligodendrogenesis and adult mPFC-related behaviours.",
author = "Anne Teissier and {Le Magueresse}, Corentin and Jimmy Olusakin and {Andrade da Costa}, {Belmira L.S.} and {De Stasi}, {Angela M.} and Alberto Bacci and {Imamura Kawasawa}, Yuka and Vaidya, {Vidita A.} and Patricia Gaspar",
year = "2019",
month = "1",
day = "1",
doi = "10.1038/s41380-019-0493-2",
language = "English (US)",
journal = "Molecular Psychiatry",
issn = "1359-4184",
publisher = "Nature Publishing Group",

}

Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms. / Teissier, Anne; Le Magueresse, Corentin; Olusakin, Jimmy; Andrade da Costa, Belmira L.S.; De Stasi, Angela M.; Bacci, Alberto; Imamura Kawasawa, Yuka; Vaidya, Vidita A.; Gaspar, Patricia.

In: Molecular Psychiatry, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Early-life stress impairs postnatal oligodendrogenesis and adult emotional behaviour through activity-dependent mechanisms

AU - Teissier, Anne

AU - Le Magueresse, Corentin

AU - Olusakin, Jimmy

AU - Andrade da Costa, Belmira L.S.

AU - De Stasi, Angela M.

AU - Bacci, Alberto

AU - Imamura Kawasawa, Yuka

AU - Vaidya, Vidita A.

AU - Gaspar, Patricia

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Exposure to stress during early life (infancy/childhood) has long-term effects on the structure and function of the prefrontal cortex (PFC), and increases the risk for adult depression and anxiety disorders. However, little is known about the molecular and cellular mechanisms of these effects. Here, we focused on changes induced by chronic maternal separation during the first 2 weeks of postnatal life. Unbiased mRNA expression profiling in the medial PFC (mPFC) of maternally separated (MS) pups identified an increased expression of myelin-related genes and a decreased expression of immediate early genes. Oligodendrocyte lineage markers and birthdating experiments indicated a precocious oligodendrocyte differentiation in the mPFC at P15, leading to a depletion of the oligodendrocyte progenitor pool in MS adults. We tested the role of neuronal activity in oligodendrogenesis, using designed receptors exclusively activated by designed drugs (DREADDs) techniques. hM4Di or hM3Dq constructs were transfected into mPFC neurons using fast-acting AAV8 viruses. Reduction of mPFC neuron excitability during the first 2 postnatal weeks caused a premature differentiation of oligodendrocytes similar to the MS pups, while chemogenetic activation normalised it in the MS animals. Bidirectional manipulation of neuron excitability in the mPFC during the P2–P14 period had long lasting effects on adult emotional behaviours and on temporal object recognition: hM4Di mimicked MS effects, while hM3Dq prevented the pro-depressive effects and short-term memory impairment of MS. Thus, our results identify neuronal activity as a critical target of early-life stress and demonstrate its function in controlling both postnatal oligodendrogenesis and adult mPFC-related behaviours.

AB - Exposure to stress during early life (infancy/childhood) has long-term effects on the structure and function of the prefrontal cortex (PFC), and increases the risk for adult depression and anxiety disorders. However, little is known about the molecular and cellular mechanisms of these effects. Here, we focused on changes induced by chronic maternal separation during the first 2 weeks of postnatal life. Unbiased mRNA expression profiling in the medial PFC (mPFC) of maternally separated (MS) pups identified an increased expression of myelin-related genes and a decreased expression of immediate early genes. Oligodendrocyte lineage markers and birthdating experiments indicated a precocious oligodendrocyte differentiation in the mPFC at P15, leading to a depletion of the oligodendrocyte progenitor pool in MS adults. We tested the role of neuronal activity in oligodendrogenesis, using designed receptors exclusively activated by designed drugs (DREADDs) techniques. hM4Di or hM3Dq constructs were transfected into mPFC neurons using fast-acting AAV8 viruses. Reduction of mPFC neuron excitability during the first 2 postnatal weeks caused a premature differentiation of oligodendrocytes similar to the MS pups, while chemogenetic activation normalised it in the MS animals. Bidirectional manipulation of neuron excitability in the mPFC during the P2–P14 period had long lasting effects on adult emotional behaviours and on temporal object recognition: hM4Di mimicked MS effects, while hM3Dq prevented the pro-depressive effects and short-term memory impairment of MS. Thus, our results identify neuronal activity as a critical target of early-life stress and demonstrate its function in controlling both postnatal oligodendrogenesis and adult mPFC-related behaviours.

UR - http://www.scopus.com/inward/record.url?scp=85071372308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071372308&partnerID=8YFLogxK

U2 - 10.1038/s41380-019-0493-2

DO - 10.1038/s41380-019-0493-2

M3 - Article

C2 - 31439936

AN - SCOPUS:85071372308

JO - Molecular Psychiatry

JF - Molecular Psychiatry

SN - 1359-4184

ER -