Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis

Xiu hua Shen, Wu feng Cheng, Xuan hai Li, Liang min Xie, Jianqin Sun, Feng Li, Lin Ma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4. METHODS: Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry. RESULT: During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little. These data confirmed that HSCs had the core effect on liver fibrogenesis and apoptosis may be a major factor regulating HSCs numbers during the injury-fibrosis-recovery sequence. At each time point, the number of activated HSCs in pathological group is more than intervention group, while apoptotic HSCs are less, which suggested dietary supplement with antioxidative nutrients had effect on HSC apoptosis but more studies are necessary to make the mechanism clearer. CONCLUSION: Dietary supplement with proper vitamin E and selenium can effectively lighten the hepatic fibrosis and promote the recovery of hepatocyte and the degradation of the existing collagens, ie, it is beneficial to the recovery of hepatic fibrosis.

Original languageEnglish (US)
Pages (from-to)194-196
Number of pages3
JournalWei sheng yan jiu = Journal of hygiene research
Volume34
Issue number2
StatePublished - 2005

Fingerprint

Hepatic Stellate Cells
Liver Cirrhosis
Fibrosis
Dietary Supplements
Antioxidants
Cell Proliferation
Apoptosis
Liver
Selenium
Vitamin E
Smooth Muscle
Actins
Collagen
Immunohistochemistry
Staining and Labeling
Uridine Triphosphate
DNA Nucleotidylexotransferase
Intraperitoneal Injections
Hepatocytes
Food

Cite this

Shen, Xiu hua ; Cheng, Wu feng ; Li, Xuan hai ; Xie, Liang min ; Sun, Jianqin ; Li, Feng ; Ma, Lin. / Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis. In: Wei sheng yan jiu = Journal of hygiene research. 2005 ; Vol. 34, No. 2. pp. 194-196.
@article{de7fbee2acea4a14a30bb632a913fe04,
title = "Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis",
abstract = "OBJECTIVE: Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4. METHODS: Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry. RESULT: During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little. These data confirmed that HSCs had the core effect on liver fibrogenesis and apoptosis may be a major factor regulating HSCs numbers during the injury-fibrosis-recovery sequence. At each time point, the number of activated HSCs in pathological group is more than intervention group, while apoptotic HSCs are less, which suggested dietary supplement with antioxidative nutrients had effect on HSC apoptosis but more studies are necessary to make the mechanism clearer. CONCLUSION: Dietary supplement with proper vitamin E and selenium can effectively lighten the hepatic fibrosis and promote the recovery of hepatocyte and the degradation of the existing collagens, ie, it is beneficial to the recovery of hepatic fibrosis.",
author = "Shen, {Xiu hua} and Cheng, {Wu feng} and Li, {Xuan hai} and Xie, {Liang min} and Jianqin Sun and Feng Li and Lin Ma",
year = "2005",
language = "English (US)",
volume = "34",
pages = "194--196",
journal = "Wei sheng yan jiu = Journal of hygiene research",
issn = "1000-8020",
publisher = "Wei sheng yan jiu{"} bian ji bu",
number = "2",

}

Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis. / Shen, Xiu hua; Cheng, Wu feng; Li, Xuan hai; Xie, Liang min; Sun, Jianqin; Li, Feng; Ma, Lin.

In: Wei sheng yan jiu = Journal of hygiene research, Vol. 34, No. 2, 2005, p. 194-196.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of antioxidant on hepatic stellate cell proliferation and apoptosis during recovery from liver fibrosis

AU - Shen, Xiu hua

AU - Cheng, Wu feng

AU - Li, Xuan hai

AU - Xie, Liang min

AU - Sun, Jianqin

AU - Li, Feng

AU - Ma, Lin

PY - 2005

Y1 - 2005

N2 - OBJECTIVE: Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4. METHODS: Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry. RESULT: During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little. These data confirmed that HSCs had the core effect on liver fibrogenesis and apoptosis may be a major factor regulating HSCs numbers during the injury-fibrosis-recovery sequence. At each time point, the number of activated HSCs in pathological group is more than intervention group, while apoptotic HSCs are less, which suggested dietary supplement with antioxidative nutrients had effect on HSC apoptosis but more studies are necessary to make the mechanism clearer. CONCLUSION: Dietary supplement with proper vitamin E and selenium can effectively lighten the hepatic fibrosis and promote the recovery of hepatocyte and the degradation of the existing collagens, ie, it is beneficial to the recovery of hepatic fibrosis.

AB - OBJECTIVE: Effects of dietary supplementation of vitamin E and selenium on proliferation and apoptosis of activated hepatic stellate cell(HSC) were investigated in the rat model of liver fibrosis induced by intraperitoneal injection with CCl4. METHODS: Activated HSC was determined by alpha-smooth muscle actin immunohistochemistry staining and apoptotic HSC determined by dual staining both of the terminal deoxynucleotidyl transferase UTP nick end labeling(TUNEL) and of alpha-smooth muscle actin immunohistochemistry. RESULT: During fibrosis recovery, the number of activated HSCs both in pathological group and in intervention group went down gradually,meanwhile, both the number of apoptotic HSCs and the collagen liver also descend little by little. These data confirmed that HSCs had the core effect on liver fibrogenesis and apoptosis may be a major factor regulating HSCs numbers during the injury-fibrosis-recovery sequence. At each time point, the number of activated HSCs in pathological group is more than intervention group, while apoptotic HSCs are less, which suggested dietary supplement with antioxidative nutrients had effect on HSC apoptosis but more studies are necessary to make the mechanism clearer. CONCLUSION: Dietary supplement with proper vitamin E and selenium can effectively lighten the hepatic fibrosis and promote the recovery of hepatocyte and the degradation of the existing collagens, ie, it is beneficial to the recovery of hepatic fibrosis.

UR - http://www.scopus.com/inward/record.url?scp=33748580724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748580724&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 194

EP - 196

JO - Wei sheng yan jiu = Journal of hygiene research

JF - Wei sheng yan jiu = Journal of hygiene research

SN - 1000-8020

IS - 2

ER -