Effect of blocking ovulation in the rat by pentobarbital on ovarian 3β-hydroxysteroid dehydrogenases

A histochemical study

V. K.K. Prabhu, Judith Weisz

Research output: Contribution to journalArticle

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Abstract

The histochemical reaction for Δ6-3β-ol-steroid dehydrogenase (3βOHD) and 3β-hydroxysteroid dehydrogenase, using a variety of substrates, was studied in ovaries of rats after spontaneous ovulation had been blocked by sodium pentobarbital for 1, 2 or 3 days. In animals that failed to ovulate, the granulosa of preovulatory type of follicles, adjacent to the theca, had increased enzyme activity after the first day of blockade and remained unchanged thereafter. The reaction of the granulosa cells closer to the antrum remained unchanged, showing either weak or no enzyme activity. This portion of the granulosa disappeared after the second day of blockade. As the blockade of ovulation continued, the size of the preovulatory type of follicles increased. Finally, changes suggesting atresia appeared in the region between the theca and the granulosa of the preovulatory follicles. There was no visible change in enzyme activity in any other ovarian structure. The degree of increase in 3/3OHD activity in the granulosa of the blocked preovulatory type of follicle was comparable for all the substrates that gave a positive reaction in the proestrous ovary. The substrate giving positive reactions were 5-androsten-3β-ol-17-one, 5-androsten-3β, 17α- diol, 5-androsten-3β, 17β-diol, 5-pregnen-3β-ol- 20-one, 5-pregnen-3β, 20α-diol and 5β-androstan- 3β-ol-17-one. No reaction was observed in either the blocked or the normal ovary when 20β3- dihydropregnenolone, 17α-hydroxypregnenolone, 5β-androstan-3α-ol-17-one, DHEA sulfate or pregnenolone sulfate were used as substrates.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
JournalEndocrinology
Volume87
Issue number3
DOIs
StatePublished - Jan 1 1970

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3-Hydroxysteroid Dehydrogenases
Pentobarbital
Ovulation
Ovary
Oxidoreductases
Enzymes
17-alpha-Hydroxypregnenolone
Steroids
Dehydroepiandrosterone Sulfate
Dehydroepiandrosterone
Granulosa Cells
androstan-3-ol

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

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title = "Effect of blocking ovulation in the rat by pentobarbital on ovarian 3β-hydroxysteroid dehydrogenases: A histochemical study",
abstract = "The histochemical reaction for Δ6-3β-ol-steroid dehydrogenase (3βOHD) and 3β-hydroxysteroid dehydrogenase, using a variety of substrates, was studied in ovaries of rats after spontaneous ovulation had been blocked by sodium pentobarbital for 1, 2 or 3 days. In animals that failed to ovulate, the granulosa of preovulatory type of follicles, adjacent to the theca, had increased enzyme activity after the first day of blockade and remained unchanged thereafter. The reaction of the granulosa cells closer to the antrum remained unchanged, showing either weak or no enzyme activity. This portion of the granulosa disappeared after the second day of blockade. As the blockade of ovulation continued, the size of the preovulatory type of follicles increased. Finally, changes suggesting atresia appeared in the region between the theca and the granulosa of the preovulatory follicles. There was no visible change in enzyme activity in any other ovarian structure. The degree of increase in 3/3OHD activity in the granulosa of the blocked preovulatory type of follicle was comparable for all the substrates that gave a positive reaction in the proestrous ovary. The substrate giving positive reactions were 5-androsten-3β-ol-17-one, 5-androsten-3β, 17α- diol, 5-androsten-3β, 17β-diol, 5-pregnen-3β-ol- 20-one, 5-pregnen-3β, 20α-diol and 5β-androstan- 3β-ol-17-one. No reaction was observed in either the blocked or the normal ovary when 20β3- dihydropregnenolone, 17α-hydroxypregnenolone, 5β-androstan-3α-ol-17-one, DHEA sulfate or pregnenolone sulfate were used as substrates.",
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Effect of blocking ovulation in the rat by pentobarbital on ovarian 3β-hydroxysteroid dehydrogenases : A histochemical study. / Prabhu, V. K.K.; Weisz, Judith.

In: Endocrinology, Vol. 87, No. 3, 01.01.1970, p. 481-485.

Research output: Contribution to journalArticle

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