Effect of cimetidine or ranitidine pretreatment on hepatic mixed function oxidase activity in the rat

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study compared the effect of single equimolar oral doses of cimetidine (100 mg/kg) or ranitidine (139 mg/kg) on rat hepatic mixed function oxidases. Cimetidine significantly (p < 0.05) increased hexobarbital sleeping time and prolonged aminopyrine and theophylline elimination. In contrast, ranitidine did not significantly affect hexobarbital sleeping time and theophylline elimination but significantly (p < 0.025) increased aminopyrine elimination. Aminopyrine N-demethylase activity in vitro was significantly (p < 0.05) inhibited by cimetidine pretreatment but significantly (p < 0.025) increased by ranitidine pretreatment. The direct addition of cimetidine or SKF 525A to the 10,000g supernatant fraction from controlled liver homogenates decreased aminopyrine N-demethylase activity, whereas the direct addition of ranitidine tended to increase aminopyrine N-demethylase activity. A significant correlation (r = 0.65, p ≤ 0.005) was observed between hexobarbital sleeping time in vivo and aminopyrine N-demethylase activity in vitro in the same rat. The results of this study showed that cimetidine inhibited mixed function oxidases, whereas ranitidine had no effect or tended to stimulate mixed function oxidases.

Original languageEnglish (US)
Pages (from-to)580-584
Number of pages5
JournalDrug Metabolism and Disposition
Volume14
Issue number5
StatePublished - Dec 1 1986

Fingerprint

Aminopyrine N-Demethylase
Ranitidine
Cimetidine
Mixed Function Oxygenases
Hexobarbital
Liver
Aminopyrine
Theophylline
Proadifen

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{8341bf6af8a64ee1871a4441da5621e3,
title = "Effect of cimetidine or ranitidine pretreatment on hepatic mixed function oxidase activity in the rat",
abstract = "This study compared the effect of single equimolar oral doses of cimetidine (100 mg/kg) or ranitidine (139 mg/kg) on rat hepatic mixed function oxidases. Cimetidine significantly (p < 0.05) increased hexobarbital sleeping time and prolonged aminopyrine and theophylline elimination. In contrast, ranitidine did not significantly affect hexobarbital sleeping time and theophylline elimination but significantly (p < 0.025) increased aminopyrine elimination. Aminopyrine N-demethylase activity in vitro was significantly (p < 0.05) inhibited by cimetidine pretreatment but significantly (p < 0.025) increased by ranitidine pretreatment. The direct addition of cimetidine or SKF 525A to the 10,000g supernatant fraction from controlled liver homogenates decreased aminopyrine N-demethylase activity, whereas the direct addition of ranitidine tended to increase aminopyrine N-demethylase activity. A significant correlation (r = 0.65, p ≤ 0.005) was observed between hexobarbital sleeping time in vivo and aminopyrine N-demethylase activity in vitro in the same rat. The results of this study showed that cimetidine inhibited mixed function oxidases, whereas ranitidine had no effect or tended to stimulate mixed function oxidases.",
author = "Yee, {Nelson Shu-Sang} and L. Shargel",
year = "1986",
month = "12",
day = "1",
language = "English (US)",
volume = "14",
pages = "580--584",
journal = "Drug Metabolism and Disposition",
issn = "0090-9556",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "5",

}

Effect of cimetidine or ranitidine pretreatment on hepatic mixed function oxidase activity in the rat. / Yee, Nelson Shu-Sang; Shargel, L.

In: Drug Metabolism and Disposition, Vol. 14, No. 5, 01.12.1986, p. 580-584.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of cimetidine or ranitidine pretreatment on hepatic mixed function oxidase activity in the rat

AU - Yee, Nelson Shu-Sang

AU - Shargel, L.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - This study compared the effect of single equimolar oral doses of cimetidine (100 mg/kg) or ranitidine (139 mg/kg) on rat hepatic mixed function oxidases. Cimetidine significantly (p < 0.05) increased hexobarbital sleeping time and prolonged aminopyrine and theophylline elimination. In contrast, ranitidine did not significantly affect hexobarbital sleeping time and theophylline elimination but significantly (p < 0.025) increased aminopyrine elimination. Aminopyrine N-demethylase activity in vitro was significantly (p < 0.05) inhibited by cimetidine pretreatment but significantly (p < 0.025) increased by ranitidine pretreatment. The direct addition of cimetidine or SKF 525A to the 10,000g supernatant fraction from controlled liver homogenates decreased aminopyrine N-demethylase activity, whereas the direct addition of ranitidine tended to increase aminopyrine N-demethylase activity. A significant correlation (r = 0.65, p ≤ 0.005) was observed between hexobarbital sleeping time in vivo and aminopyrine N-demethylase activity in vitro in the same rat. The results of this study showed that cimetidine inhibited mixed function oxidases, whereas ranitidine had no effect or tended to stimulate mixed function oxidases.

AB - This study compared the effect of single equimolar oral doses of cimetidine (100 mg/kg) or ranitidine (139 mg/kg) on rat hepatic mixed function oxidases. Cimetidine significantly (p < 0.05) increased hexobarbital sleeping time and prolonged aminopyrine and theophylline elimination. In contrast, ranitidine did not significantly affect hexobarbital sleeping time and theophylline elimination but significantly (p < 0.025) increased aminopyrine elimination. Aminopyrine N-demethylase activity in vitro was significantly (p < 0.05) inhibited by cimetidine pretreatment but significantly (p < 0.025) increased by ranitidine pretreatment. The direct addition of cimetidine or SKF 525A to the 10,000g supernatant fraction from controlled liver homogenates decreased aminopyrine N-demethylase activity, whereas the direct addition of ranitidine tended to increase aminopyrine N-demethylase activity. A significant correlation (r = 0.65, p ≤ 0.005) was observed between hexobarbital sleeping time in vivo and aminopyrine N-demethylase activity in vitro in the same rat. The results of this study showed that cimetidine inhibited mixed function oxidases, whereas ranitidine had no effect or tended to stimulate mixed function oxidases.

UR - http://www.scopus.com/inward/record.url?scp=0022922637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022922637&partnerID=8YFLogxK

M3 - Article

C2 - 2876865

AN - SCOPUS:0022922637

VL - 14

SP - 580

EP - 584

JO - Drug Metabolism and Disposition

JF - Drug Metabolism and Disposition

SN - 0090-9556

IS - 5

ER -