Effect of conformation and drop properties on surface-enhanced Raman spectroscopy of dried biopolymer drops

Karen A. Esmonde-White, Stephanie Valerie Le Clair, Blake J. Roessler, Michael D. Morris

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Biofluids are complex solutions consisting of small ions and large biopolymers such as DNA, proteins, or proteoglycans. Biopolymers affect fluid properties but their effect on drop deposition has not been examined. Hyaluronic acid (HA), an important component in synovial fluid, was chosen as a model biopolymer, and examined using surface-enhanced Raman spectroscopy (SERS). Nanoliter volumes of HA solutions were dried onto a patterned SERS substrate and spectra were collected from the dried hyaluronic acid drops with a near-infrared Raman microscope. Characteristic hyaluronic acid bands were examined. Capillary viscometry measured properties of HA solutions, and entanglement behavior was also modeled using scaling theory principles. Viscosity measurements were incorporated into models of suspended particle droplets to account for the effect of inter-chain attraction on droplet formation. Microscope images were used to evaluate the shape of the dried drop. Relative drop thickness was estimated from concentric rings found at drop edges using established models of light interference by thin films. We found SERS spectra were sensitive not only to polymer conformation, but also to type of deposition (ring versus uniform), and the thickness of the resulting deposition. These data suggest an approach to elucidate the effects of biopolymers and dehydrated biofluids on SERS analysis.

Original languageEnglish (US)
Pages (from-to)503-511
Number of pages9
JournalApplied Spectroscopy
Volume62
Issue number5
DOIs
StatePublished - May 1 2008

Fingerprint

Biopolymers
biopolymers
Hyaluronic acid
Raman spectroscopy
Conformations
Hyaluronic Acid
acids
Viscosity measurement
microscopes
viscometry
Microscopes
fluids
rings
Light interference
attraction
Fluids
deoxyribonucleic acid
Proteoglycans
viscosity
proteins

All Science Journal Classification (ASJC) codes

  • Spectroscopy
  • Instrumentation

Cite this

Esmonde-White, Karen A. ; Le Clair, Stephanie Valerie ; Roessler, Blake J. ; Morris, Michael D. / Effect of conformation and drop properties on surface-enhanced Raman spectroscopy of dried biopolymer drops. In: Applied Spectroscopy. 2008 ; Vol. 62, No. 5. pp. 503-511.
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Effect of conformation and drop properties on surface-enhanced Raman spectroscopy of dried biopolymer drops. / Esmonde-White, Karen A.; Le Clair, Stephanie Valerie; Roessler, Blake J.; Morris, Michael D.

In: Applied Spectroscopy, Vol. 62, No. 5, 01.05.2008, p. 503-511.

Research output: Contribution to journalArticle

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