Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards

M. França, R. Poulson, Justin David Brown, E. W. Howerth, R. D. Berghaus, D. Carter, D. E. Stallknecht

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We studied the effect of different routes of inoculation on the infectivity and duration of viral shedding in mallards (Anas platyrhynchos) infected with wild bird-origin low pathogenic avian influenza viruses (LPAIVs). One-month-old mallards were inoculated with 106 median embryo infectious doses of either A/mallard/MN/199106/99 (H3N8) or A/mallard/MN/355779/00 (H5N2) via 1 of 5 different routes: intranasal (IN), intratracheal (IT), intraocular (IO), intracloacal (IC), or intra-ingluvial (II). Birds in all routes of inoculation groups became infected with LPAIV as detected by virus isolation, real time reverse transcription polymerase chain reaction, and serology. Mallards in different route of inoculation groups had similar viral shedding through oropharynx and cloaca from 1 day postinoculation (dpi). The peak of oropharyngeal (OP) viral shedding was reached between 2 and 3 dpi in all routes of inoculation groups infected with either virus. The peak of cloacal (CL) viral excretion was reached between 2 and 3 dpi in all routes of inoculation groups infected with H3N8 LPAIV and in the IO-, IC-, and II-inoculated groups infected with H5N2 LPAIV, with a delayed and shorter peak for the IN-and IT-inoculated groups. The birds inoculated via the II route had more productive OP and CL viral shedding after infection with either LPAIV, as evidenced by higher number of swabs testing positive over the study period. In conclusion, mallards can be infected with LPAIV by various routes of inoculation, and this corroborates their high susceptibility to infection by these viruses.

Original languageEnglish (US)
Pages (from-to)981-985
Number of pages5
JournalAvian diseases
Volume56
Issue number4 SUPPL.1
DOIs
StatePublished - Dec 1 2012

Fingerprint

Virus Shedding
viral shedding
Influenza in Birds
Anas platyrhynchos
Orthomyxoviridae
Influenza A virus
pathogenicity
Viruses
viruses
Birds
Cloaca
Oropharynx
Virus Diseases
Serology
cloaca
birds
wild birds
Reverse Transcription
infection
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

França, M., Poulson, R., Brown, J. D., Howerth, E. W., Berghaus, R. D., Carter, D., & Stallknecht, D. E. (2012). Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards. Avian diseases, 56(4 SUPPL.1), 981-985. https://doi.org/10.1637/10151-040812-ResNote.1
França, M. ; Poulson, R. ; Brown, Justin David ; Howerth, E. W. ; Berghaus, R. D. ; Carter, D. ; Stallknecht, D. E. / Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards. In: Avian diseases. 2012 ; Vol. 56, No. 4 SUPPL.1. pp. 981-985.
@article{dbefa914c16241a083b5b79b5e19da13,
title = "Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards",
abstract = "We studied the effect of different routes of inoculation on the infectivity and duration of viral shedding in mallards (Anas platyrhynchos) infected with wild bird-origin low pathogenic avian influenza viruses (LPAIVs). One-month-old mallards were inoculated with 106 median embryo infectious doses of either A/mallard/MN/199106/99 (H3N8) or A/mallard/MN/355779/00 (H5N2) via 1 of 5 different routes: intranasal (IN), intratracheal (IT), intraocular (IO), intracloacal (IC), or intra-ingluvial (II). Birds in all routes of inoculation groups became infected with LPAIV as detected by virus isolation, real time reverse transcription polymerase chain reaction, and serology. Mallards in different route of inoculation groups had similar viral shedding through oropharynx and cloaca from 1 day postinoculation (dpi). The peak of oropharyngeal (OP) viral shedding was reached between 2 and 3 dpi in all routes of inoculation groups infected with either virus. The peak of cloacal (CL) viral excretion was reached between 2 and 3 dpi in all routes of inoculation groups infected with H3N8 LPAIV and in the IO-, IC-, and II-inoculated groups infected with H5N2 LPAIV, with a delayed and shorter peak for the IN-and IT-inoculated groups. The birds inoculated via the II route had more productive OP and CL viral shedding after infection with either LPAIV, as evidenced by higher number of swabs testing positive over the study period. In conclusion, mallards can be infected with LPAIV by various routes of inoculation, and this corroborates their high susceptibility to infection by these viruses.",
author = "M. Fran{\cc}a and R. Poulson and Brown, {Justin David} and Howerth, {E. W.} and Berghaus, {R. D.} and D. Carter and Stallknecht, {D. E.}",
year = "2012",
month = "12",
day = "1",
doi = "10.1637/10151-040812-ResNote.1",
language = "English (US)",
volume = "56",
pages = "981--985",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "4 SUPPL.1",

}

França, M, Poulson, R, Brown, JD, Howerth, EW, Berghaus, RD, Carter, D & Stallknecht, DE 2012, 'Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards', Avian diseases, vol. 56, no. 4 SUPPL.1, pp. 981-985. https://doi.org/10.1637/10151-040812-ResNote.1

Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards. / França, M.; Poulson, R.; Brown, Justin David; Howerth, E. W.; Berghaus, R. D.; Carter, D.; Stallknecht, D. E.

In: Avian diseases, Vol. 56, No. 4 SUPPL.1, 01.12.2012, p. 981-985.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards

AU - França, M.

AU - Poulson, R.

AU - Brown, Justin David

AU - Howerth, E. W.

AU - Berghaus, R. D.

AU - Carter, D.

AU - Stallknecht, D. E.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - We studied the effect of different routes of inoculation on the infectivity and duration of viral shedding in mallards (Anas platyrhynchos) infected with wild bird-origin low pathogenic avian influenza viruses (LPAIVs). One-month-old mallards were inoculated with 106 median embryo infectious doses of either A/mallard/MN/199106/99 (H3N8) or A/mallard/MN/355779/00 (H5N2) via 1 of 5 different routes: intranasal (IN), intratracheal (IT), intraocular (IO), intracloacal (IC), or intra-ingluvial (II). Birds in all routes of inoculation groups became infected with LPAIV as detected by virus isolation, real time reverse transcription polymerase chain reaction, and serology. Mallards in different route of inoculation groups had similar viral shedding through oropharynx and cloaca from 1 day postinoculation (dpi). The peak of oropharyngeal (OP) viral shedding was reached between 2 and 3 dpi in all routes of inoculation groups infected with either virus. The peak of cloacal (CL) viral excretion was reached between 2 and 3 dpi in all routes of inoculation groups infected with H3N8 LPAIV and in the IO-, IC-, and II-inoculated groups infected with H5N2 LPAIV, with a delayed and shorter peak for the IN-and IT-inoculated groups. The birds inoculated via the II route had more productive OP and CL viral shedding after infection with either LPAIV, as evidenced by higher number of swabs testing positive over the study period. In conclusion, mallards can be infected with LPAIV by various routes of inoculation, and this corroborates their high susceptibility to infection by these viruses.

AB - We studied the effect of different routes of inoculation on the infectivity and duration of viral shedding in mallards (Anas platyrhynchos) infected with wild bird-origin low pathogenic avian influenza viruses (LPAIVs). One-month-old mallards were inoculated with 106 median embryo infectious doses of either A/mallard/MN/199106/99 (H3N8) or A/mallard/MN/355779/00 (H5N2) via 1 of 5 different routes: intranasal (IN), intratracheal (IT), intraocular (IO), intracloacal (IC), or intra-ingluvial (II). Birds in all routes of inoculation groups became infected with LPAIV as detected by virus isolation, real time reverse transcription polymerase chain reaction, and serology. Mallards in different route of inoculation groups had similar viral shedding through oropharynx and cloaca from 1 day postinoculation (dpi). The peak of oropharyngeal (OP) viral shedding was reached between 2 and 3 dpi in all routes of inoculation groups infected with either virus. The peak of cloacal (CL) viral excretion was reached between 2 and 3 dpi in all routes of inoculation groups infected with H3N8 LPAIV and in the IO-, IC-, and II-inoculated groups infected with H5N2 LPAIV, with a delayed and shorter peak for the IN-and IT-inoculated groups. The birds inoculated via the II route had more productive OP and CL viral shedding after infection with either LPAIV, as evidenced by higher number of swabs testing positive over the study period. In conclusion, mallards can be infected with LPAIV by various routes of inoculation, and this corroborates their high susceptibility to infection by these viruses.

UR - http://www.scopus.com/inward/record.url?scp=84871963695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871963695&partnerID=8YFLogxK

U2 - 10.1637/10151-040812-ResNote.1

DO - 10.1637/10151-040812-ResNote.1

M3 - Article

C2 - 23402123

AN - SCOPUS:84871963695

VL - 56

SP - 981

EP - 985

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 4 SUPPL.1

ER -

França M, Poulson R, Brown JD, Howerth EW, Berghaus RD, Carter D et al. Effect of different routes of inoculation on infectivity and viral shedding of LPAI viruses in mallards. Avian diseases. 2012 Dec 1;56(4 SUPPL.1):981-985. https://doi.org/10.1637/10151-040812-ResNote.1