Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds

Abril Fonseca-García, Josué D. Mota-Morales, Iraís A. Quintero-Ortega, Zaira Y. García-Carvajal, V. Martínez-Lõpez, Erika Ruvalcaba, Carlos Landa-Solís, Lilia Solis, Clemente Ibarra, María C. Gutiérrez, Mauricio Terrones, Isaac C. Sanchez, Francisco Del Monte, María C. Velasquillo, G. Luna-Bárcenas

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This work describes the preparation and characterization of biomimetic chitosan/multiwall carbon nanotubes/nano-hydroxyapatite (CTS/MWCNT/nHAp) scaffolds and their viability for bone tissue engineering applications. The cryogenic process ice segregation-induced self-assembly (ISISA) was used to fabricate 3D biomimetic CTS scaffolds. Proper combination of cryogenics, freeze-drying, nature and molecular ratio of solutes give rise to 3D porous interconnected scaffolds with clusters of nHAp distributed along the scaffold surface. The effect of doping in CNT (e.g. with oxygen and nitrogen atoms) on cell viability was tested. Under the same processing conditions, pore size was in the range of 20-150 μm and irrespective on the type of CNT. Studies on cell viability with scaffolds were carried out using human cells from periosteum biopsy. Prior to cell seeding, the immunophenotype of mesenchymal periosteum or periosteum-derived stem cells (MSCs-PCs) was characterized by flow cytometric analysis using fluorescence-activated and characteristic cell surface markers for MSCs-PCs. The characterized MSCs-PCs maintained their periosteal potential in cell cultures until the 2nd passage from primary cell culture. Thus, the biomimetic CTS/MWCNT/nHAp scaffolds demonstrated good biocompatibility and cell viability in all cases such that it can be considered as promising biomaterials for bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)3341-3351
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number10
DOIs
StatePublished - Oct 2014

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Carbon Nanotubes
Chitosan
Biomimetics
Durapatite
Scaffolds (biology)
Hydroxyapatite
Carbon nanotubes
Cells
Doping (additives)
Scaffolds
Tissue engineering
Cell culture
Cryogenics
Bone
Biopsy
Ice
Biocompatible Materials
Stem cells
Biocompatibility
Biomaterials

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Fonseca-García, A., Mota-Morales, J. D., Quintero-Ortega, I. A., García-Carvajal, Z. Y., Martínez-Lõpez, V., Ruvalcaba, E., ... Luna-Bárcenas, G. (2014). Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds. Journal of Biomedical Materials Research - Part A, 102(10), 3341-3351. https://doi.org/10.1002/jbm.a.34893
Fonseca-García, Abril ; Mota-Morales, Josué D. ; Quintero-Ortega, Iraís A. ; García-Carvajal, Zaira Y. ; Martínez-Lõpez, V. ; Ruvalcaba, Erika ; Landa-Solís, Carlos ; Solis, Lilia ; Ibarra, Clemente ; Gutiérrez, María C. ; Terrones, Mauricio ; Sanchez, Isaac C. ; Del Monte, Francisco ; Velasquillo, María C. ; Luna-Bárcenas, G. / Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds. In: Journal of Biomedical Materials Research - Part A. 2014 ; Vol. 102, No. 10. pp. 3341-3351.
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abstract = "This work describes the preparation and characterization of biomimetic chitosan/multiwall carbon nanotubes/nano-hydroxyapatite (CTS/MWCNT/nHAp) scaffolds and their viability for bone tissue engineering applications. The cryogenic process ice segregation-induced self-assembly (ISISA) was used to fabricate 3D biomimetic CTS scaffolds. Proper combination of cryogenics, freeze-drying, nature and molecular ratio of solutes give rise to 3D porous interconnected scaffolds with clusters of nHAp distributed along the scaffold surface. The effect of doping in CNT (e.g. with oxygen and nitrogen atoms) on cell viability was tested. Under the same processing conditions, pore size was in the range of 20-150 μm and irrespective on the type of CNT. Studies on cell viability with scaffolds were carried out using human cells from periosteum biopsy. Prior to cell seeding, the immunophenotype of mesenchymal periosteum or periosteum-derived stem cells (MSCs-PCs) was characterized by flow cytometric analysis using fluorescence-activated and characteristic cell surface markers for MSCs-PCs. The characterized MSCs-PCs maintained their periosteal potential in cell cultures until the 2nd passage from primary cell culture. Thus, the biomimetic CTS/MWCNT/nHAp scaffolds demonstrated good biocompatibility and cell viability in all cases such that it can be considered as promising biomaterials for bone tissue engineering.",
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Fonseca-García, A, Mota-Morales, JD, Quintero-Ortega, IA, García-Carvajal, ZY, Martínez-Lõpez, V, Ruvalcaba, E, Landa-Solís, C, Solis, L, Ibarra, C, Gutiérrez, MC, Terrones, M, Sanchez, IC, Del Monte, F, Velasquillo, MC & Luna-Bárcenas, G 2014, 'Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds', Journal of Biomedical Materials Research - Part A, vol. 102, no. 10, pp. 3341-3351. https://doi.org/10.1002/jbm.a.34893

Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds. / Fonseca-García, Abril; Mota-Morales, Josué D.; Quintero-Ortega, Iraís A.; García-Carvajal, Zaira Y.; Martínez-Lõpez, V.; Ruvalcaba, Erika; Landa-Solís, Carlos; Solis, Lilia; Ibarra, Clemente; Gutiérrez, María C.; Terrones, Mauricio; Sanchez, Isaac C.; Del Monte, Francisco; Velasquillo, María C.; Luna-Bárcenas, G.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 10, 10.2014, p. 3341-3351.

Research output: Contribution to journalArticle

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AU - Fonseca-García, Abril

AU - Mota-Morales, Josué D.

AU - Quintero-Ortega, Iraís A.

AU - García-Carvajal, Zaira Y.

AU - Martínez-Lõpez, V.

AU - Ruvalcaba, Erika

AU - Landa-Solís, Carlos

AU - Solis, Lilia

AU - Ibarra, Clemente

AU - Gutiérrez, María C.

AU - Terrones, Mauricio

AU - Sanchez, Isaac C.

AU - Del Monte, Francisco

AU - Velasquillo, María C.

AU - Luna-Bárcenas, G.

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N2 - This work describes the preparation and characterization of biomimetic chitosan/multiwall carbon nanotubes/nano-hydroxyapatite (CTS/MWCNT/nHAp) scaffolds and their viability for bone tissue engineering applications. The cryogenic process ice segregation-induced self-assembly (ISISA) was used to fabricate 3D biomimetic CTS scaffolds. Proper combination of cryogenics, freeze-drying, nature and molecular ratio of solutes give rise to 3D porous interconnected scaffolds with clusters of nHAp distributed along the scaffold surface. The effect of doping in CNT (e.g. with oxygen and nitrogen atoms) on cell viability was tested. Under the same processing conditions, pore size was in the range of 20-150 μm and irrespective on the type of CNT. Studies on cell viability with scaffolds were carried out using human cells from periosteum biopsy. Prior to cell seeding, the immunophenotype of mesenchymal periosteum or periosteum-derived stem cells (MSCs-PCs) was characterized by flow cytometric analysis using fluorescence-activated and characteristic cell surface markers for MSCs-PCs. The characterized MSCs-PCs maintained their periosteal potential in cell cultures until the 2nd passage from primary cell culture. Thus, the biomimetic CTS/MWCNT/nHAp scaffolds demonstrated good biocompatibility and cell viability in all cases such that it can be considered as promising biomaterials for bone tissue engineering.

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Fonseca-García A, Mota-Morales JD, Quintero-Ortega IA, García-Carvajal ZY, Martínez-Lõpez V, Ruvalcaba E et al. Effect of doping in carbon nanotubes on the viability of biomimetic chitosan-carbon nanotubes-hydroxyapatite scaffolds. Journal of Biomedical Materials Research - Part A. 2014 Oct;102(10):3341-3351. https://doi.org/10.1002/jbm.a.34893