Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae

J. L. Isaacs, E. Vresilovic, M. Marcolongo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Intervertebral disc (IVD) degeneration is associated with changes in the major biomolecular components in the disc as well as changes in enzymatic activity in the IVD. Collagen, elastin, and proteoglycans in the disc are primarily responsible for mechanical and structural support as well as disc recovery after loading. In order to investigate the effects of the loss of these biomolecules, a single annulus fibrosus (AF) lamellar model has been developed. Isolated segments of human AF were digested with elastase, collagenase and chondroitinase ABC to selectively degenerate collagen, elastin, and proteoglycan respectively. Samples were then mechanically tested to determine the contribution of these components to the mechanical properties. Understanding the effects of biomolecular changes in the IVD will promote understanding of the relationship between composition and mechanical behavior.

Original languageEnglish (US)
Title of host publicationProceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
DOIs
StatePublished - Jun 8 2010
Event36th Annual Northeast Bioengineering Conference, NEBEC 2010 - New York, NY, United States
Duration: Mar 26 2010Mar 28 2010

Publication series

NameProceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

Other

Other36th Annual Northeast Bioengineering Conference, NEBEC 2010
CountryUnited States
CityNew York, NY
Period3/26/103/28/10

Fingerprint

Mechanical properties
Elastin
Proteoglycans
Collagen
Chondroitin ABC Lyase
Pancreatic Elastase
Biomolecules
Collagenases
Recovery
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Isaacs, J. L., Vresilovic, E., & Marcolongo, M. (2010). Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae. In Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010 [5458160] (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010). https://doi.org/10.1109/NEBC.2010.5458160
Isaacs, J. L. ; Vresilovic, E. ; Marcolongo, M. / Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae. Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010. 2010. (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010).
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abstract = "Intervertebral disc (IVD) degeneration is associated with changes in the major biomolecular components in the disc as well as changes in enzymatic activity in the IVD. Collagen, elastin, and proteoglycans in the disc are primarily responsible for mechanical and structural support as well as disc recovery after loading. In order to investigate the effects of the loss of these biomolecules, a single annulus fibrosus (AF) lamellar model has been developed. Isolated segments of human AF were digested with elastase, collagenase and chondroitinase ABC to selectively degenerate collagen, elastin, and proteoglycan respectively. Samples were then mechanically tested to determine the contribution of these components to the mechanical properties. Understanding the effects of biomolecular changes in the IVD will promote understanding of the relationship between composition and mechanical behavior.",
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Isaacs, JL, Vresilovic, E & Marcolongo, M 2010, Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae. in Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010., 5458160, Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010, 36th Annual Northeast Bioengineering Conference, NEBEC 2010, New York, NY, United States, 3/26/10. https://doi.org/10.1109/NEBC.2010.5458160

Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae. / Isaacs, J. L.; Vresilovic, E.; Marcolongo, M.

Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010. 2010. 5458160 (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Isaacs JL, Vresilovic E, Marcolongo M. Effect of enzymatic digestion on the transverse mechanical properties of annulus fibrosus lamellae. In Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010. 2010. 5458160. (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010). https://doi.org/10.1109/NEBC.2010.5458160