Effect of glycerol and cholesterol-loaded cyclodextrin on freezing-induced water loss in bovine spermatozoa

G. Li, J. Saenz, R. A. Godke, R. V. Devireddy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recent experimental data show that incubating bovine sperm with cholesterol-loaded cyclodextrin (CLC) before cryopreservation increases the percentages of motile and viable cells recovered after freezing and thawing, compared with control sperm. In the present study, we report the effect of incubating bovine sperm with CLC on the subzero water transport response and the membrane permeability parameters (reference membrane permeability (Lpg) and activation energy (ELp)). Water transport data during freezing of bovine sperm cell suspensions were obtained at a cooling rate of 20 °C/min under three different conditions: 1. in the absence of cryoprotective agents (CPAs); 2. in the presence of 0.7 M glycerol; and 3. in the presence of 1.5 mg/ml CLC and 0.7 M glycerol. With previously published values, the bovine sperm cell was modeled as a cylinder of length 39.8 μm and radius 0.4 μm, with osmotically inactive cell volume (Vb) of 0.61 Vo, where Vo is the isotonic cell volume. By fitting a model of water transport to the experimentally obtained data, the best-fit water transport parameters (Lpg and ELp) were determined. The predicted best-fit permeability parameters ranged from Lpg = 0.02 to 0.036 μm/min-atm and ELP = 26.4 to 42.1 kcal/mol. These subzero water transport parameters are significantly different from the suprazero membrane permeability values (obtained in the absence of extracellular ice) reported in the literature. Calculations made of the theoretical response of bovine spermatozoa at subzero temperatures suggest that the optimal cooling rate to cryopreserve bovine spermatozoa is 45-60 °C/min, agreeing quite closely with experimentally determined rates of freezing bovine spermatozoa.

Original languageEnglish (US)
Pages (from-to)875-886
Number of pages12
JournalReproduction
Volume131
Issue number5
DOIs
StatePublished - May 1 2006

Fingerprint

Cyclodextrins
Glycerol
Freezing
Spermatozoa
Cholesterol
Water
Permeability
Cell Size
Membranes
Cryoprotective Agents
Cryopreservation
Ice
Suspensions
Temperature

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Cite this

Li, G. ; Saenz, J. ; Godke, R. A. ; Devireddy, R. V. / Effect of glycerol and cholesterol-loaded cyclodextrin on freezing-induced water loss in bovine spermatozoa. In: Reproduction. 2006 ; Vol. 131, No. 5. pp. 875-886.
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Effect of glycerol and cholesterol-loaded cyclodextrin on freezing-induced water loss in bovine spermatozoa. / Li, G.; Saenz, J.; Godke, R. A.; Devireddy, R. V.

In: Reproduction, Vol. 131, No. 5, 01.05.2006, p. 875-886.

Research output: Contribution to journalArticle

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