Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes

Ricard Bou, Francesc Guardiola, Rafael Codony, Cameron Faustman, Ryan J. Elias, Eric A. Decker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Myoglobin (Mb) and its iron have been proposed to be major prooxidants in cooked meats. To understand the mechanisms and differentiate between the prooxidant and antioxidant potential of oxymyoglobin (OxyMb) and metmyoglobin (MetMb), their prooxidant activity, iron content, solubility, free radical scavenging activity, and iron binding capacity were determined as a function of thermal processing. The ability of native and heat denatured OxyMb and MetMb to promote the oxidation of muscle microsomes was different. MetMb promoted lipid oxidation in both its native and denatured states. Conversely, OxyMb became antioxidative when the protein was heated to temperatures ≥75 °C. The increased antioxidant activity of heat denatured OxyMb was likely due to a decrease in its prooxidative activity due to its loss of solubility. These data show that the impact on oxidative reactions of Mb is the result of the balance between its antioxidant and prooxidant activities.

Original languageEnglish (US)
Pages (from-to)9612-9620
Number of pages9
JournalJournal of agricultural and food chemistry
Volume56
Issue number20
DOIs
StatePublished - Oct 22 2008

Fingerprint

Metmyoglobin
microsomes
Microsomes
Heating
Muscle
myoglobin
oxidation
iron
heat
Muscles
Oxidation
muscles
Iron
solubility
Antioxidants
Hot Temperature
Myoglobin
Solubility
antioxidants
binding capacity

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Bou, Ricard ; Guardiola, Francesc ; Codony, Rafael ; Faustman, Cameron ; Elias, Ryan J. ; Decker, Eric A. / Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes. In: Journal of agricultural and food chemistry. 2008 ; Vol. 56, No. 20. pp. 9612-9620.
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Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes. / Bou, Ricard; Guardiola, Francesc; Codony, Rafael; Faustman, Cameron; Elias, Ryan J.; Decker, Eric A.

In: Journal of agricultural and food chemistry, Vol. 56, No. 20, 22.10.2008, p. 9612-9620.

Research output: Contribution to journalArticle

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T1 - Effect of heating oxymyoglobin and metmyoglobin on the oxidation of muscle microsomes

AU - Bou, Ricard

AU - Guardiola, Francesc

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