Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.

Translated title of the contribution: Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro

N. I. Sakharova, I. A. Kostanian, T. N. Lepikhova, K. A. Lepikhov, A. M. Malashenko, E. V. Navolotskaia, J. Tombran-Tink, V. M. Lipkin

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Abstract

We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.

Original languageRussian
Pages (from-to)195-200
Number of pages6
JournalOntogenez
Volume33
Issue number3
Publication statusPublished - Jan 1 2002

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Sakharova, N. I., Kostanian, I. A., Lepikhova, T. N., Lepikhov, K. A., Malashenko, A. M., Navolotskaia, E. V., ... Lipkin, V. M. (2002). Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro. Ontogenez, 33(3), 195-200.