Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval asian longhorned beetle

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Anoplophora glabripennis, the Asian longhorned beetle, is a wood-boring insect that can develop in a wide range of healthy deciduous hosts and requires gut microbes to aid in wood degradation and digestion. Here we show that larval A. glabripennis harbor a diverse gut bacterial community, and this community can be extremely variable when reared in different host trees. A. glabripennis reared in a preferred host (Acer saccharum) had the highest gut bacterial diversity compared with larvae reared either in a secondary host (Quercus palustris), a resistant host (Pyrus calleryana), or on artificial diet. The gut microbial community of larval A. glabripennis collected from field populations on Brooklyn, NY, showed the highest degree of complexity among all samples in this study. Overall, when larvae fed on a preferred host, they harbored a broad diversity of gut bacteria spanning the α-, β-, γ- Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Cellulase activities (β-1,4-endoglucanase, β-1,4-exoglucanase, and β-1,4-glucosidase) in the guts of larvae fed in a preferred host (A. saccharum) or a secondary host (Q. palustris) were significantly higher than that of artificial diet fed larvae. Larvae that fed on wood from a resistant host (P. calleryana) showed suppressed total gut cellulase activity. Results show that the host tree can impact both gut microbial community complexity and cellulase activity in A. glabripennis.

Original languageEnglish (US)
Pages (from-to)686-699
Number of pages14
JournalEnvironmental Entomology
Volume38
Issue number3
DOIs
StatePublished - Jun 2009

Fingerprint

Anoplophora glabripennis
endo-1,4-beta-glucanase
bacterial communities
community composition
beetle
digestive system
Pyrus calleryana
Quercus palustris
larvae
larva
Acer saccharum
intermediate hosts
artificial diets
intestinal microorganisms
artificial diet
microbial communities
microbial community
glucosidases
Firmicutes
decayed wood

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Insect Science

Cite this

@article{15a26d9e41b64204ad0b174ee33c50c3,
title = "Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval asian longhorned beetle",
abstract = "Anoplophora glabripennis, the Asian longhorned beetle, is a wood-boring insect that can develop in a wide range of healthy deciduous hosts and requires gut microbes to aid in wood degradation and digestion. Here we show that larval A. glabripennis harbor a diverse gut bacterial community, and this community can be extremely variable when reared in different host trees. A. glabripennis reared in a preferred host (Acer saccharum) had the highest gut bacterial diversity compared with larvae reared either in a secondary host (Quercus palustris), a resistant host (Pyrus calleryana), or on artificial diet. The gut microbial community of larval A. glabripennis collected from field populations on Brooklyn, NY, showed the highest degree of complexity among all samples in this study. Overall, when larvae fed on a preferred host, they harbored a broad diversity of gut bacteria spanning the α-, β-, γ- Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Cellulase activities (β-1,4-endoglucanase, β-1,4-exoglucanase, and β-1,4-glucosidase) in the guts of larvae fed in a preferred host (A. saccharum) or a secondary host (Q. palustris) were significantly higher than that of artificial diet fed larvae. Larvae that fed on wood from a resistant host (P. calleryana) showed suppressed total gut cellulase activity. Results show that the host tree can impact both gut microbial community complexity and cellulase activity in A. glabripennis.",
author = "Geib, {Scott M.} and {Jimenez Gasco}, {Maria Del Mar} and Carlson, {John Edward} and Ming Tien and Kelli Hoover",
year = "2009",
month = "6",
doi = "10.1603/022.038.0320",
language = "English (US)",
volume = "38",
pages = "686--699",
journal = "Environmental Entomology",
issn = "0046-225X",
publisher = "Entomological Society of America",
number = "3",

}

TY - JOUR

T1 - Effect of host tree species on cellulase activity and bacterial community composition in the gut of larval asian longhorned beetle

AU - Geib, Scott M.

AU - Jimenez Gasco, Maria Del Mar

AU - Carlson, John Edward

AU - Tien, Ming

AU - Hoover, Kelli

PY - 2009/6

Y1 - 2009/6

N2 - Anoplophora glabripennis, the Asian longhorned beetle, is a wood-boring insect that can develop in a wide range of healthy deciduous hosts and requires gut microbes to aid in wood degradation and digestion. Here we show that larval A. glabripennis harbor a diverse gut bacterial community, and this community can be extremely variable when reared in different host trees. A. glabripennis reared in a preferred host (Acer saccharum) had the highest gut bacterial diversity compared with larvae reared either in a secondary host (Quercus palustris), a resistant host (Pyrus calleryana), or on artificial diet. The gut microbial community of larval A. glabripennis collected from field populations on Brooklyn, NY, showed the highest degree of complexity among all samples in this study. Overall, when larvae fed on a preferred host, they harbored a broad diversity of gut bacteria spanning the α-, β-, γ- Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Cellulase activities (β-1,4-endoglucanase, β-1,4-exoglucanase, and β-1,4-glucosidase) in the guts of larvae fed in a preferred host (A. saccharum) or a secondary host (Q. palustris) were significantly higher than that of artificial diet fed larvae. Larvae that fed on wood from a resistant host (P. calleryana) showed suppressed total gut cellulase activity. Results show that the host tree can impact both gut microbial community complexity and cellulase activity in A. glabripennis.

AB - Anoplophora glabripennis, the Asian longhorned beetle, is a wood-boring insect that can develop in a wide range of healthy deciduous hosts and requires gut microbes to aid in wood degradation and digestion. Here we show that larval A. glabripennis harbor a diverse gut bacterial community, and this community can be extremely variable when reared in different host trees. A. glabripennis reared in a preferred host (Acer saccharum) had the highest gut bacterial diversity compared with larvae reared either in a secondary host (Quercus palustris), a resistant host (Pyrus calleryana), or on artificial diet. The gut microbial community of larval A. glabripennis collected from field populations on Brooklyn, NY, showed the highest degree of complexity among all samples in this study. Overall, when larvae fed on a preferred host, they harbored a broad diversity of gut bacteria spanning the α-, β-, γ- Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Cellulase activities (β-1,4-endoglucanase, β-1,4-exoglucanase, and β-1,4-glucosidase) in the guts of larvae fed in a preferred host (A. saccharum) or a secondary host (Q. palustris) were significantly higher than that of artificial diet fed larvae. Larvae that fed on wood from a resistant host (P. calleryana) showed suppressed total gut cellulase activity. Results show that the host tree can impact both gut microbial community complexity and cellulase activity in A. glabripennis.

UR - http://www.scopus.com/inward/record.url?scp=68049110286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68049110286&partnerID=8YFLogxK

U2 - 10.1603/022.038.0320

DO - 10.1603/022.038.0320

M3 - Article

C2 - 19508777

AN - SCOPUS:68049110286

VL - 38

SP - 686

EP - 699

JO - Environmental Entomology

JF - Environmental Entomology

SN - 0046-225X

IS - 3

ER -